Murohashi I, Tohda S, Suzuki T, Nagata K, Yamashita Y, Nara N
First Department of Internal Medicine, Tokyo Medical and Dental University, Japan.
Exp Hematol. 1990 Feb;18(2):133-7.
We tested the effect of interleukin 1 (IL-1) on the growth of leukemic blast progenitors from patients with acute myeloblastic leukemia (AML). A purified blast cell fraction depleted of both T cells and phagocytic cells was tested at different cell densities. Addition of 1 ng/ml of IL-1 alpha alone enhanced blast colony formation in 10 of 13 cases tested, and the enhancement was prominent when plated cell densities were lowered. The conditioned media (CM) from AML patients contained varied levels of IL-1 activity, and following depletion of phagocytic cells, the levels decreased markedly in all cases tested. Addition of either antiserum against IL-1 alpha or IL-1 beta reduced the IL-1 activity in CM, suggesting that AML blasts produce both IL-1 alpha and IL-1 beta. Addition of IL-1 alpha or IL-1 beta antiserum inhibited blast colony formation in a dose-dependent manner, and a combination of both antisera showed the most marked inhibition. However, the augmentation of blast colony formation was almost completely inhibited by addition of anti-granulocyte-macrophage colony-stimulating factor (GM-CSF) serum in all three cases tested. IL-1 is also devoid of this activity when tested in the presence of a combination of granulocyte CSF (G-CSF), GM-CSF, and interleukin 3 (IL-3) at an optimal concentration. These results suggest that blast cells could produce and secrete CSF(s) and/or IL-1, and that the growth-enhancing effect of IL-1 on AML blasts is indirect, via production of CSFs by leukemic cells.
我们检测了白细胞介素1（IL-1）对急性髓细胞白血病（AML）患者白血病原始祖细胞生长的影响。对去除T细胞和吞噬细胞的纯化原始细胞组分在不同细胞密度下进行检测。单独添加1 ng/ml的IL-1α可增强13例受试病例中10例的原始细胞集落形成，且当接种细胞密度降低时这种增强作用更为显著。AML患者的条件培养基（CM）含有不同水平的IL-1活性，在去除吞噬细胞后，所有受试病例中的水平均显著下降。添加抗IL-1α或IL-1β抗血清可降低CM中的IL-1活性，表明AML原始细胞可产生IL-1α和IL-1β。添加IL-1α或IL-1β抗血清可剂量依赖性地抑制原始细胞集落形成，两种抗血清联合使用时抑制作用最为明显。然而，在所有3例受试病例中，添加抗粒细胞-巨噬细胞集落刺激因子（GM-CSF）血清几乎完全抑制了原始细胞集落形成的增强。在最佳浓度的粒细胞集落刺激因子（G-CSF）、GM-CSF和白细胞介素3（IL-3）联合存在的情况下进行检测时，IL-1也没有这种活性。这些结果表明，原始细胞可产生并分泌集落刺激因子（CSF）和/或IL-1，且IL-1对AML原始细胞的生长促进作用是间接的，通过白血病细胞产生CSF来实现。
