[胃癌中HER2蛋白表达及基因扩增分析]

[Analysis of the protein expression and gene amplification of HER2 in gastric cancer].

作者信息

Liu Jun-lin, Li Zhen-kai, Xin Fang-jie, Zhao Han, Zhao Wei, Gao Yuan, Hu Ji-lin, Zhang Xian-xiang, Zhang Mao-shen, Zhang Bing-yuan, Lu Yun

机构信息

Department of General Surgery, Huangdao Branch, Affiliated Hospital, Qingdao University Medical College, Qingdao 266555, China.

出版信息

Zhonghua Wei Chang Wai Ke Za Zhi. 2013 Sep;16(9):898-901.

PMID:24062004

Abstract

OBJECTIVE

To explore the clinical significance of protein expression and gene amplification of HER2 in gastric cancer.

METHODS

Immunohistochemistry (IHC) and chromogenic in situ hybridization (CISH) method were used to detect protein expression and gene amplification of HER2 in 80 specimens of gastric cancer patients.

RESULTS

Protein expression of HER2 was negative in 51 cases, (+) in 12 cases, (++) in 12 cases, (+++) in 5 cases, and the positive expression rate was 21.3% (17/80). Seven (8.8%) cases had gene amplification of HER2, including gene critical amplification in 3 (3.8%) cases. The result of IHC was positively correlated with CISH (P<0.05), and the coincidence rate was 85.0% (68/80). HER2 positive expression rate was higher in the gastroesophageal junction carcinoma, poorly differentiated and stage III-IIII gastric cancer (all P<0.05).

CONCLUSION

The gastric cancer tissue has high positive rate of protein expression and gene amplification of HER2, which is closely correlated to the development of gastric cancer.

摘要

目的

探讨人表皮生长因子受体2（HER2）蛋白表达及基因扩增在胃癌中的临床意义。

方法

采用免疫组织化学（IHC）和显色原位杂交（CISH）方法检测80例胃癌患者标本中HER2的蛋白表达及基因扩增情况。

结果

HER2蛋白表达阴性51例，（+）12例，（++）12例，（+++）5例，阳性表达率为21.3%（17/80）。7例（8.8%）存在HER2基因扩增，其中3例（3.8%）为基因临界扩增。IHC结果与CISH结果呈正相关（P<0.05），符合率为85.0%（68/80）。胃食管交界癌、低分化及Ⅲ-Ⅳ期胃癌的HER2阳性表达率较高（均P<0.05）。