Hardin S E, Weaver R F
Department of Biochemistry, University of Kansas, Lawrence 66045.
J Gen Virol. 1990 Jan;71 ( Pt 1):225-9. doi: 10.1099/0022-1317-71-1-225.
We have mapped a set of transcripts that cross the XhoI site in the HindIII F fragment of the genome of the Autographa californica nuclear polyhedrosis virus. These transcripts overlap at their 5' ends by about 550 bases and run in opposite directions. We have tentatively identified two open reading frames corresponding to the leftward transcript. The rightward transcript is present from 8 h post-infection (p.i.) to 24 h p.i.; the leftward transcripts are present from 2 h p.i. to 24 h p.i. The early (2 h) transcript is about 2.1 kb in size and its 5' end maps about 504 bp to the right of the XhoI site. Beginning at about 8 h p.i. a new transcription start site is used, about 80 bp downstream (to the left) of the first. The late (rightward) transcript is about 1.2 kb in size; its 5' end seems to be heterogeneous and maps about 44 to 60 bp to the left of the XhoI site. Late in infection transcription proceeds in both directions at the same time through the overlapping region of the DNA encoding these transcripts.
我们已经绘制了一组转录本图谱，这些转录本跨越了苜蓿银纹夜蛾核型多角体病毒基因组HindIII F片段中的XhoI位点。这些转录本在其5'端重叠约550个碱基，并且方向相反。我们初步鉴定出了与向左转录本相对应的两个开放阅读框。向右转录本在感染后8小时（p.i.）至24小时p.i.存在；向左转录本在感染后2小时p.i.至24小时p.i.存在。早期（2小时）转录本大小约为2.1 kb，其5'端位于XhoI位点右侧约504 bp处。从感染后约8小时开始，使用了一个新的转录起始位点，位于第一个起始位点下游（向左）约80 bp处。晚期（向右）转录本大小约为1.2 kb；其5'端似乎具有异质性，位于XhoI位点左侧约44至60 bp处。在感染后期，转录同时在两个方向上通过编码这些转录本的DNA重叠区域进行。
