Ding H F, Nakoneczna I, Hsu H S
Department of Microbiology and Immunology, Medical College of Virginia, Virginia Commonwealth University, Richmond 23298.
J Med Microbiol. 1990 Feb;31(2):95-102. doi: 10.1099/00222615-31-2-95.
C3H/HeNMTV mice were immunised intraperitoneally (i.p.) with lipopolysaccharide (LPS) or detoxified LPS (D-LPS) derived from Salmonella typhimurium strain SR-11. In both cases, effective protection was achieved against a challenge dose of greater than 2 x 10(2) LD50 of the same organism given by i.p. injection. However, by comparison with LPS, approximately 6- to 10-fold more of D-LPS by weight was needed to protect mice to an equivalent degree. Histopathological studies showed that the initial lesions in infected mice protected with either LPS or D-LPS were composed of self-limiting abscesses which transformed into granulomas as the animals recovered. It is suggested that D-LPS may be modified to become a highly effective, non-toxic salmonella vaccine.
用源自鼠伤寒沙门氏菌菌株SR-11的脂多糖（LPS）或解毒脂多糖（D-LPS）对C3H/HeNMTV小鼠进行腹腔内（i.p.）免疫。在这两种情况下，通过腹腔注射给予大于2×10² LD50的相同生物体的攻击剂量时，均实现了有效保护。然而，与LPS相比，按重量计需要大约6至10倍的D-LPS才能使小鼠得到同等程度的保护。组织病理学研究表明，用LPS或D-LPS保护的感染小鼠的初始病变由自限性脓肿组成，随着动物恢复，这些脓肿会转变为肉芽肿。有人提出，D-LPS可以进行修饰，成为一种高效、无毒的沙门氏菌疫苗。
