Institute of First Clinical Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210046, P.R. China.
Oncol Rep. 2013 Dec;30(6):2923-8. doi: 10.3892/or.2013.2749. Epub 2013 Sep 20.
In the present study, we investigated the antitumor activity of caudatin in the human hepatoma cell line SMMC‑7721 by analysis of cell viability, cell cycle distribution, apoptosis and metastasis. The results showed that caudatin impaired the cell viability and inhibited the growth of SMMC-7721 cells in a time- and dose-dependent manner and resulted in cell cycle arrest in the G2 phase. In addition, SMMC-7721 cells, treated with caudatin exhibited typical characteristics of apoptosis. Furthermore, caudatin treatment resulted in a decrease in β-catenin and GSK3β in SMMC-7721 cells, with a concomitant reduction in metastatic capability and expression of Wnt signaling pathway targeted genes including cox-2, mmp-2 and mmp-9. Our findings revealed that caudatin inhibits human hepatoma cell growth and metastasis by targeting the GSK3β/β-catenin pathway and suppressing VEGF production.
在本研究中，我们通过分析细胞活力、细胞周期分布、细胞凋亡和转移，研究了北美蟾毒精在人肝癌细胞系 SMMC-7721 中的抗肿瘤活性。结果表明，北美蟾毒精可损害细胞活力，抑制 SMMC-7721 细胞的生长，呈时间和剂量依赖性，并导致细胞周期停滞在 G2 期。此外，用北美蟾毒精处理 SMMC-7721 细胞后，可观察到典型的细胞凋亡特征。此外，北美蟾毒精处理可导致 SMMC-7721 细胞中β-catenin 和 GSK3β 的减少，同时降低转移能力以及 Wnt 信号通路靶向基因包括 cox-2、mmp-2 和 mmp-9 的表达。我们的研究结果表明，北美蟾毒精通过靶向 GSK3β/β-catenin 通路和抑制 VEGF 的产生，抑制人肝癌细胞的生长和转移。
