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脊尾白虾(Exopalaemon carinicauda)多态性微卫星位点的分离与特征分析

Isolation and characterization of polymorphic microsatellite loci in the ridgetail white prawn Exopalaemon carinicauda.

作者信息

Jia S W, Liu P, Li J, Li J T, Pan L Q

机构信息

Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong, China.

出版信息

Genet Mol Res. 2013 Aug 8;12(3):2816-20. doi: 10.4238/2013.August.8.1.

Abstract

The ridgetail white prawn Exopalaemon carinicauda is one of the major economic shrimp species cultured in China. In this study, 30 microsatellite loci were isolated and characterized for the ridgetail white prawn E. carinicauda using a microsatellite-enriched library. Polymorphisms were tested in 30 individuals from a single wild population. The number of alleles at each locus ranged from 2 to 14. The observed and expected heterozygosities varied from 0.1000 to 0.8000 and from 0.2299 to 0.9228, respectively. The PIC value ranged from 0.2002 to 0.8939. These new loci will be useful in the study of population genetic structure and genetic diversity in this species.

摘要

脊尾白虾(Exopalaemon carinicauda)是中国主要的养殖经济虾类之一。在本研究中,利用微卫星富集文库为脊尾白虾分离并鉴定了30个微卫星位点。在来自单个野生群体的30个个体中检测了多态性。每个位点的等位基因数从2到14不等。观察到的杂合度和期望杂合度分别在0.1000至0.8000和0.2299至0.9228之间。PIC值在0.2002至0.8939之间。这些新位点将有助于该物种的群体遗传结构和遗传多样性研究。

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