Elliott G, Ferguson L, Everts R, Edwards R
Pathology Services, Otago Area Health Board, Dunedin.
N Z Med J. 1990 Jan 24;103(882):13-6.
Two approaches to mutagenicity monitoring were compared for estimation of possible absorption of cytotoxic drugs by a group of six pharmacists at Auckland Hospital. Blood samples were taken before and after a three week duty roster, from age and sex matched negative controls, and from a matched group of patients on chemotherapy. Peripheral blood lymphocytes were fixed, stained and analysed for sister chromatid exchange (SCE). Urine samples were collected from the pharmacists on the morning following their last day on cytotoxic dispensing duty, as well as from positive and negative controls. Samples were concentrated and analysed for mutagenicity in the bacterial Ames test. Comparison of negative control groups with the pharmacists using either of these test measures gave no evidence for significant absorption of cytotoxics by the pharmacist group as a whole, although values for the positive control groups were raised significantly in a similar comparison. The value for SCE of one pharmacist however, was elevated in the second sample (after three weeks dispensing duty) when compared with the first sample (before duty) and the urine analysis also suggested that this individual might have absorbed some mutagens. The results permit a comparison of these techniques as a means of ongoing monitoring of similar populations within New Zealand.
为评估奥克兰医院的六名药剂师对细胞毒性药物的可能吸收情况，比较了两种监测致突变性的方法。在为期三周的值班排班前后，采集了年龄和性别匹配的阴性对照以及一组接受化疗的匹配患者的血样。对固定、染色后的外周血淋巴细胞进行姐妹染色单体交换（SCE）分析。在药剂师最后一天进行细胞毒性药物调配工作后的早晨，收集了他们的尿样，以及阳性和阴性对照的尿样。将样品浓缩后，在细菌艾姆斯试验中分析其致突变性。使用这两种测试方法中的任何一种，将阴性对照组与药剂师组进行比较，均未发现药剂师组整体对细胞毒性药物有明显吸收的证据，尽管在类似比较中阳性对照组的值显著升高。然而，与第一个样品（值班前）相比，一名药剂师在第二个样品（三周值班后）中的SCE值升高，尿液分析也表明该个体可能吸收了一些诱变剂。这些结果有助于比较这些技术，作为新西兰对类似人群进行持续监测的一种手段。
