Department of Zoology, College of Science, Osmania University, Hyderabad 500 007, A. P., India.
Z Naturforsch C J Biosci. 2013 Jul-Aug;68(7-8):318-26.
The sublethal stress of the organophosphate insecticide chlorpyrifos was investigated in different tissues of the freshwater crab (Barytelphusa guerini). Crabs were exposed to 1/3 of LC50 concentrations for 7, 14, 21, and 28 days. After 28 days, they were released into fresh water and kept for 18 days for recovery. The study was conducted by estimating total proteins, amino acids, ammonia, urea, and glutamine levels, and protease, transaminases, and phosphatases activities. Total proteins level was decreased whereas amino acids and ammonia were increased. The urea content was decreased in all tissues and glutamine exhibited a mixed response. Protease activities and those of alanine and aspartate aminotransferase, respectively, were elevated. Acid phosphatase activity was reduced in hepatopancreas and brain and induced in gills and muscle. Alkaline phosphatase activity was enhanced in gills and hepatopancreas and reduced in muscle and brain. The crabs recovered from the biochemical stress caused by chlorpyrifos after their release into fresh water.
有机磷杀虫剂毒死蜱对淡水蟹（Barytelphusa guerini）不同组织的亚致死应激进行了研究。将螃蟹暴露于 LC50 浓度的 1/3 中 7、14、21 和 28 天。28 天后，它们被释放到淡水中，并保持 18 天进行恢复。通过估计总蛋白质、氨基酸、氨、尿素和谷氨酰胺水平以及蛋白酶、转氨酶和磷酸酶活性来进行研究。总蛋白质水平降低，而氨基酸和氨增加。所有组织中的尿素含量降低，谷氨酰胺表现出混合反应。蛋白酶活性以及丙氨酸和天冬氨酸转氨酶的活性分别升高。酸性磷酸酶在肝胰腺和脑中的活性降低，在鳃和肌肉中诱导。碱性磷酸酶在鳃和肝胰腺中的活性增强，在肌肉和脑中降低。螃蟹在被释放到淡水中后，从由毒死蜱引起的生化应激中恢复过来。
