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Generation of deletion derivatives by targeted transformation of human-derived yeast artificial chromosomes.

作者信息

Pavan W J, Hieter P, Reeves R H

机构信息

Department of Physiology, Johns Hopkins University School of Medicine, Baltimore, MD 21205.

出版信息

Proc Natl Acad Sci U S A. 1990 Feb;87(4):1300-4. doi: 10.1073/pnas.87.4.1300.

DOI:10.1073/pnas.87.4.1300
PMID:2406718
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC53462/
Abstract

Mammalian DNA segments cloned as yeast artificial chromosomes (YACs) can be manipulated by DNA-mediated transformation when placed in an appropriate yeast genetic background. A "fragmenting vector" has been developed that can introduce a yeast telomere and selectable marker into human-derived YACs at specific sites by means of homologous recombination, deleting all sequences distal to the recombination site. A powerful application of the method uses a human Alu family repeat sequence to target recombination to multiple independent sites on a human-derived YAC. Sets of deletion derivatives generated by this procedure greatly facilitate restriction mapping of large genomic segments. Targeting recombination with single copy sequences, such as cDNAs, will have many additional applications. This approach establishes a paradigm for manipulation and characterization of mammalian DNA segments cloned as YACs.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d54/53462/a27220a8bf44/pnas01029-0053-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d54/53462/51d72f382be7/pnas01029-0051-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d54/53462/9e77a4dd54f3/pnas01029-0051-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d54/53462/5b07b3e00b09/pnas01029-0052-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d54/53462/136585a6bef2/pnas01029-0052-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d54/53462/a27220a8bf44/pnas01029-0053-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d54/53462/51d72f382be7/pnas01029-0051-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d54/53462/9e77a4dd54f3/pnas01029-0051-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d54/53462/5b07b3e00b09/pnas01029-0052-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d54/53462/136585a6bef2/pnas01029-0052-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d54/53462/a27220a8bf44/pnas01029-0053-a.jpg

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本文引用的文献

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Separation of chromosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresis.通过正交交变电场凝胶电泳从酵母中分离染色体DNA分子。
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Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.通过脉冲场梯度凝胶电泳分离酵母染色体大小的DNA。
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Transformation of intact yeast cells treated with alkali cations.经碱金属阳离子处理的完整酵母细胞的转化
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Regulation of the myeloid-cell-expressed human gp91-phox gene as studied by transfer of yeast artificial chromosome clones into embryonic stem cells: suppression of a variegated cellular pattern of expression requires a full complement of distant cis elements.通过将酵母人工染色体克隆转移到胚胎干细胞中来研究髓样细胞表达的人类gp91-噬氧细胞色素基因的调控：抑制斑驳的细胞表达模式需要完整的远距离顺式元件。
Mol Cell Biol. 1997 Apr;17(4):2279-90. doi: 10.1128/MCB.17.4.2279.
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A shuttle system for transfer of YACs between yeast and mammalian cells.一种用于在酵母细胞和哺乳动物细胞之间转移酵母人工染色体（YAC）的穿梭系统。
Nucleic Acids Res. 1996 Dec 1;24(23):4693-9. doi: 10.1093/nar/24.23.4693.
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Patterns of meiotic double-strand breakage on native and artificial yeast chromosomes.天然和人工酵母染色体上减数分裂双链断裂的模式。
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Yeast recombination: the association between double-strand gap repair and crossing-over.酵母重组：双链缺口修复与交叉互换之间的关联。
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Base sequence studies of 300 nucleotide renatured repeated human DNA clones.300个核苷酸的复性重复人类DNA克隆的碱基序列研究。
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Yeast transformation: a model system for the study of recombination.酵母转化：用于重组研究的模型系统。
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Cell. 1985 Feb;40(2):381-92. doi: 10.1016/0092-8674(85)90152-7.