Plant Physiology and Biotechnology Division, UPASI Tea Research Institute, Valparai, 642127, Tamilnadu, India.
Appl Biochem Biotechnol. 2014 Jan;172(1):216-23. doi: 10.1007/s12010-013-0529-z. Epub 2013 Sep 26.
Reactive oxygen species (ROS) production is the first level of response by a host during stress. Even though the ROS are toxic to cell, when present in a limited amount, they act as a signalling molecule for the expression of defence-related genes and later are scavenged by either enzymatic or non-enzymatic mechanisms of the host. The different anti-oxidative enzymes like glutathione reductase (GR), superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APO), peroxidase (POD) and polyphenol oxidase (PPO) were estimated, and their activities were compared between infected and healthy leaves of the tolerant and susceptible cultivars of tea. The infected leaves of the susceptible cultivars registered higher amount of enzyme activity when compared with the tolerant cultivars. The study reveals that the more anti-oxidative enzymes, the more susceptible the cultivar will be.
活性氧(ROS)的产生是宿主在应激时的第一级反应。尽管 ROS 对细胞有毒,但当它们处于有限的数量时,它们就会作为一种信号分子,来表达防御相关基因,随后被宿主的酶或非酶机制清除。不同的抗氧化酶,如谷胱甘肽还原酶(GR)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APO)、过氧化物酶(POD)和多酚氧化酶(PPO),都被进行了评估,并比较了其在耐受和易感茶树品种的健康和感染叶片中的活性。与耐受品种相比,易感品种的感染叶片中的酶活性更高。该研究表明,抗氧化酶越多,品种就越易感。