Department of Oriental Gynecology, College of Korean Medicine, Kyung Hee University, No. 1 Hoegi-dong, Dongdaemun-gu, Seoul 130-701, Republic of Korea.
Evid Based Complement Alternat Med. 2013;2013:976270. doi: 10.1155/2013/976270. Epub 2013 Aug 31.
Dangguijakyak-san (DJS), a famous traditional Korean multiherbal medicine, has been used to treat gynecological and neuro-associated disease. Recent studies demonstrated that DJS has multiple bioactivities including neuroprotection. In the present study, we were to investigate the effect of DJS and its mechanism in an in vitro and in vivo model of Parkinson's disease (PD). In primary mesencephalic culture system, DJS attenuated the dopaminergic cell damage induced by 1-methyl-4-phenylpyridine toxicity, and it inhibited production of inflammatory factors such as tumor necrosis factor α (TNF- α ), nitric oxide (NO), and activation of microglial cells. Then, we confirmed the effect of DJS in a mouse PD model induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). In the pole test, DJS at 50 mg/kg/day for 5 days showed increase of motor activity showing shortened time to turn and locomotor activity compared with the MPTP only treated mice. In addition, DJS significantly protected nigrostriatal dopaminergic neuron from MPTP stress. Moreover, DJS showed inhibition of gliosis in the substantia nigra pars compacta. These results have therapeutic implications for DJS in the treatment of PD via anti-inflammatory effects.
当归芍药散（DJS）是一种著名的韩方草药，用于治疗妇科和神经相关疾病。最近的研究表明，DJS 具有多种生物活性，包括神经保护作用。在本研究中，我们旨在研究 DJS 在帕金森病（PD）的体外和体内模型中的作用及其机制。在原代中脑培养系统中，DJS 减轻了 1-甲基-4-苯基吡啶毒性诱导的多巴胺能细胞损伤，并抑制了肿瘤坏死因子 α（TNF-α）、一氧化氮（NO）等炎症因子的产生和小胶质细胞的激活。然后，我们在 1-甲基-4-苯基-1,2,3,6-四氢吡啶（MPTP）诱导的小鼠 PD 模型中证实了 DJS 的作用。在杆试验中，DJS 在 50mg/kg/天连续 5 天处理后，与仅用 MPTP 处理的小鼠相比，运动活性增加，表现为转向时间缩短和运动活性增加。此外，DJS 显著保护黑质致密部的多巴胺能神经元免受 MPTP 应激。此外，DJS 显示出对黑质中神经胶质增生的抑制作用。这些结果表明 DJS 通过抗炎作用对 PD 的治疗具有潜在的治疗意义。
