Klein J, Colin P, Scherer E, Levy M, Koren G
Department of Pediatrics, Hospital for Sick Children, Toronto, Ontario, Canada.
Ther Drug Monit. 1990 Jan;12(1):105-10. doi: 10.1097/00007691-199001000-00019.
Captopril is an orally active inhibitor of angiotensin-converting enzyme. A rapid, accurate, and sensitive high-performance chromatography (HPLC) method is described for measuring plasma concentrations of captopril. Captopril was stabilized by forming an adduct with p-bromophenacyl bromide. This adduct was measured by HPLC using a C-18 reverse-phase column and monitoring the column effluent by ultraviolet absorption at 260 nm. The method proved to be linear in the clinical range of 10-1,000 ng/ml. The plasma levels of captopril in a young patient given a small oral dose were determined by this method.
卡托普利是一种口服有效的血管紧张素转换酶抑制剂。本文描述了一种快速、准确且灵敏的高效液相色谱（HPLC）法，用于测定血浆中卡托普利的浓度。卡托普利通过与对溴苯甲酰溴形成加合物来实现稳定化。该加合物采用C-18反相柱通过HPLC进行测定，并在260nm波长处通过紫外吸收监测柱流出物。该方法在10 - 1000 ng/ml的临床范围内被证明具有线性关系。通过该方法测定了一名年轻患者口服小剂量卡托普利后的血浆水平。
