Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Corps, Fujian Agriculture and Forestry University , Shangxiadian Road No. 15, Cangshan District, Fuzhou 350002, P. R. China.
J Proteome Res. 2013 Nov 1;12(11):5124-36. doi: 10.1021/pr4006469. Epub 2013 Oct 16.
Kandelia candel is being established as a model xylophyte for ecoadaptation due to its salt tolerance. To adapt to high salinity, the photosynthesis apparatus must function efficiently under these conditions. Proteomic analysis of chloroplasts isolated from plants under different degrees of salt stress was performed to quantify the changes of individual proteins and to gain a global view of the total chloroplast protein dynamics. Among the 1030 proteins quantified (unique peptide ≥ 1), 76 showed a more than 1.5-fold change in abundance, of which 36 are involved in the light-dependent reactions and 12 in the Calvin cycle. The dynamic change of these proteins indicates that light-dependent reactions are maintained by up-regulating the levels of component proteins at both moderate and high salinity, and the Calvin cycle remained functional at moderate salinity but showed a decline at high salinity. In addition to proteins related to photosynthesis, some known abiotic-stress proteins and plastoglobuli were up-regulated in salt-stressed plants. Plastoglobuli might contribute to maintaining membrane integrity and fluidity. In conclusion, this extensive proteomic investigation on intact chloroplasts of the salt-tolerant xylophyte under salt stress provides some important novel information on adaptative mechanisms involving photosynthesis in responses to salt stress in K. candel.
由于耐盐性，秋茄（Kandelia candel）正被确立为生态适应性的模式木本植物。为了适应高盐度，光合作用器官必须在这些条件下高效运作。对不同盐度胁迫下植物分离的叶绿体进行蛋白质组学分析，以定量个别蛋白质的变化，并全面了解总叶绿体蛋白动态。在定量的 1030 种蛋白质（独特肽≥1）中，有 76 种的丰度增加了 1.5 倍以上，其中 36 种参与光依赖反应，12 种参与卡尔文循环。这些蛋白质的动态变化表明，在中度和高度盐度下，通过上调组成蛋白的水平来维持光依赖反应，卡尔文循环在中度盐度下保持功能，但在高度盐度下下降。除了与光合作用相关的蛋白质外，一些已知的非生物胁迫蛋白和质体小球也在盐胁迫下的植物中上调。质体小球可能有助于维持膜的完整性和流动性。总之，对耐盐木本植物秋茄在盐胁迫下完整叶绿体的广泛蛋白质组学研究，为盐胁迫下涉及光合作用的适应机制提供了一些重要的新信息。
