a Ningbo Academy of Inspection and Quarantine of the P.R. China , Ningbo , P.R. China.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2013;30(11):1888-99. doi: 10.1080/19440049.2013.833670. Epub 2013 Sep 26.
A quantitative multi-class analytical method covering more than 226 veterinary drugs and other contaminants in muscle, belonging to different drug families, was developed. The method is based on liquid-liquid extraction, purification by low-temperature clean-up and dispersive solid-phase extraction (D-SPE), and analysis was conducted in two analytical runs by column-switching UPLC coupled with electrospray ionisation and tandem mass spectrometry (UPLC-ESI-MS/MS). For most of the target analytes, the optimised pre-treatment processes led to no significant interference from the sample matrix. The limit of quantification varied from 0.05 to 10 µg kg(-1). Statistical evaluation indicated that average recoveries spiked into pork were in the range from 62.4% to 138.8%, and the relative standard deviations were in the range from 2.8% to 26.6% (intra-day precision). The availability of this method will contribute to a better safety assurance of meat with a significant reduction of both effort and time.
建立了一种涵盖 226 种以上兽药和其他肌肉中污染物的定量多类分析方法，这些污染物属于不同的药物家族。该方法基于液-液萃取、低温净化和分散固相萃取（D-SPE），通过柱切换超高效液相色谱-电喷雾串联质谱（UPLC-ESI-MS/MS）进行两次分析运行。对于大多数目标分析物，优化的预处理过程不会对样品基质产生明显干扰。定量限从 0.05 到 10µg/kg 不等。统计评估表明，猪肉中添加的平均回收率在 62.4%至 138.8%之间，相对标准偏差在 2.8%至 26.6%（日内精密度）之间。该方法的可用性将有助于更好地保证肉类安全，大大减少工作量和时间。
