Department of Chemical Engineering, National Taiwan University, Taipei 10617, Taiwan.
J Colloid Interface Sci. 2013 Dec 1;411:162-8. doi: 10.1016/j.jcis.2013.08.033. Epub 2013 Aug 29.
Recent advances in fabrication techniques make nano-sized pores as promising platforms for both detection and sequencing of individual biopolymers such as DNA. To simulate the electrokinetic behavior of a particle in this case, we consider the electrophoresis of a soft nanocylinder comprising a rigid core and a pH-regulated, zwitterionic polyelectrolyte layer along the axis of a rigid cylindrical nanopore. Extending the conventional electrophoresis analysis, where the liquid phase contains only one kind each of cations and anions, we assume that it contains multiple ionic species, as is usually the case in practice. The key parameters are examined for their influences on the electrokinetic behavior of a particle. These include pH, the thickness of the polyelectrolyte layer and the density of its functional groups, and the pore size. The results gathered provide necessary information for the design of an electrokinetic apparatus.
最近在制造技术方面的进展使得纳米级孔成为检测和测序单个生物聚合物（如 DNA）的有前途的平台。为了模拟这种情况下粒子的电动行为，我们考虑了沿着刚性圆柱纳米孔的轴电泳的软纳米圆柱，该纳米圆柱由刚性核和 pH 调节的两性离子聚电解质层组成。扩展传统的电泳分析，其中液相仅含有每一种阳离子和阴离子，我们假设它包含多种离子种类，这在实践中通常是这样的。检查了关键参数对粒子电动行为的影响。这些参数包括 pH 值、聚电解质层的厚度和官能团的密度以及孔径。收集的结果为电动仪器的设计提供了必要的信息。
