基于增强型金纳米粒子的乳腺癌生物标志物酶联免疫吸附测定法。

Enhanced gold nanoparticle based ELISA for a breast cancer biomarker.

机构信息

Nanobioelectronics and Biosensors Group, Institut Català de Nanotecnologia, CIN2 (ICN-CSIC), Barcelona, Catalonia, Spain.

出版信息

Anal Chem. 2010 Feb 1;82(3):1151-6. doi: 10.1021/ac902492c.

DOI:10.1021/ac902492c

PMID:20043655

Abstract

In this work, we developed an optical enzyme-linked immunosorbent assay (ELISA) immunoassay for the analysis of CA15-3 antigen, an important biomarker present in blood samples and useful for the follow-up of the medical treatment of breast cancer. Gold nanoparticles (AuNPs) were used as carriers of the signaling antibody anti-CA15-3-HRP (horseradish peroxidase) in order to achieve an amplification of the optical signal. In the range between 0 and 60 U/mL, the assay adopting AuNPs as an enhancer resulted in higher sensitivity and shorter assay time when compared to classical ELISA procedures. The application of AuNPs to the commercially available ELISA test can be useful to improve important immunoanalysis procedures where a more confident result is needed.

摘要

在这项工作中，我们开发了一种用于分析 CA15-3 抗原的光学酶联免疫吸附测定（ELISA）免疫分析方法，CA15-3 抗原是血液样本中存在的一种重要生物标志物，可用于乳腺癌治疗的随访。金纳米粒子（AuNPs）被用作信号抗体抗 CA15-3-HRP（辣根过氧化物酶）的载体，以实现光学信号的放大。在 0 到 60 U/mL 的范围内，与经典 ELISA 程序相比，采用 AuNPs 作为增强剂的测定法具有更高的灵敏度和更短的测定时间。将 AuNPs 应用于市售的 ELISA 测试可能有助于改进需要更可信结果的重要免疫分析程序。