Yurttutan Sadık, Ozdemir Ramazan, Canpolat Fuat Emre, Oncel Mehmet Yekta, Unverdi Hatice Germen, Uysal Bülent, Erdeve Ömer, Dilmen Ugur
Neonatal Intensive Care Unit, Zekai Tahir Burak Maternity Teaching Hospital, 06110, Hamamönü/Ankara, Turkey,
Pediatr Surg Int. 2014 Jan;30(1):71-7. doi: 10.1007/s00383-013-3415-4. Epub 2013 Sep 27.
Tissue damage in necrotizing enterocolitis (NEC) of infants occurs as a result of an uncontrolled inflammatory response. The aim of this study was to investigate any potential anti-inflammatory effects that Etanercept may have on the inflammatory response in an experimental NEC model in newborn rats.
Newborn pups were randomized into three groups immediately after birth (Control, NEC + Placebo and NEC + Etanercept). Pups in the NEC + Placebo and NEC + Etanercept groups were subjected to an NEC-inducing protocol (hypercarbia, hypothermia and hyperoxia) twice a day for 3 days. Pups in the NEC + Etanercept group were given an intraperitoneal injection of Etanercept. Rats were harvested for biochemical and histopathological examinations.
The histopathological injury score of rats in the NEC + Placebo group was significantly higher compared to the NEC + Etanercept and Control groups (p < 0.05 for both comparisons). Tissue levels of tumor necrosis factor-α, interleukin-1β, and malondialdehyde were higher in the placebo group compared to the Etanercept group.
Our results suggest that Etanercept attenuates intestinal tissue damage in NEC by reducing inflammation and blocking the production of free-oxygen radicals, while also reducing tissue levels of tumor necrosis factor-α and interleukin-1β.
婴儿坏死性小肠结肠炎（NEC）中的组织损伤是由不受控制的炎症反应所致。本研究的目的是探讨依那西普对新生大鼠实验性NEC模型炎症反应可能具有的抗炎作用。
新生幼崽出生后立即随机分为三组（对照组、NEC + 安慰剂组和NEC + 依那西普组）。NEC + 安慰剂组和NEC + 依那西普组的幼崽每天接受两次诱导NEC的方案（高碳酸血症、体温过低和高氧），持续3天。NEC + 依那西普组的幼崽腹腔注射依那西普。处死大鼠进行生化和组织病理学检查。
与NEC + 依那西普组和对照组相比，NEC + 安慰剂组大鼠的组织病理学损伤评分显著更高（两组比较均为p < 0.05）。与依那西普组相比，安慰剂组的肿瘤坏死因子-α、白细胞介素-1β和丙二醛的组织水平更高。
我们的结果表明，依那西普通过减轻炎症、阻断游离氧自由基的产生，同时降低肿瘤坏死因子-α和白细胞介素-1β的组织水平，减轻NEC中的肠道组织损伤。
