Department of Neonatology, Health Sciences University, Zekai Tahir Burak Maternity Education and Research Hospital, Ankara, Turkey.
Health Sciences University, Zekai Tahir Burak Maternity Education and Research Hospital, Ankara, Turkey.
J Ethnopharmacol. 2018 Oct 28;225:297-308. doi: 10.1016/j.jep.2018.07.009. Epub 2018 Jul 11.
Necrotizing enterocolitis (NEC) is the most important gastrointestinal emergency affecting especially preterm infants and causes severe morbidities and mortality. However, there is no cure. Oxidant stress, inflammation, apoptosis, as well as prematurity are believed to responsible in the pathogenesis of the disease. Ginger and its compounds have anti-inflammatory, antimicrobial, anti-oxidant properties and immunomodulatory, cytoprotective/regenerative actions.
This study aimed to evaluate the beneficial effects of ginger on the intestinal damage in an experimental rat model of NEC.
Thirty newborn Wistar rats were divided into three groups: NEC, NEC + ginger and control in this experimental study. NEC was induced by injection of intraperitoneal lipopolysaccharide, feeding with enteral formula, hypoxia-hyperoxia and cold stress exposure. The pups in the NEC + ginger group were orally administered ginger at a dose of 1000 mg/kg/day. Proximal colon and ileum were excised. Histopathological, immunohistochemical (TUNEL for apoptosis, caspase 3 and 8) and biochemical assays including xanthine oxidase (XO), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), malonaldehyde (MDA) and myeloperoxidase (MPO), tumor necrosis factor-α (TNF-α), interleukin1β (IL-1β), and interleukin 6 (IL-6) activity were evaluated.
Compared with the NEC group, the rat pups in the NEC + ginger group had better clinical disease scores and weight gain (p < 0.05). Macroscopic evaluation, Histopathologic and apoptosis assessment (TUNEL, caspase 3 and 8) releaved that severity of intestinal damage were significantly lower in the NEC + ginger group (p < 0.05). The levels of TNF-α, IL-1β and IL-6 in the ginger treated group were significantly decreased (P < 0.05). The GSH-Px and SOD levels of the ginger treated group were significantly preserved in the NEC + ginger group (p < 0.05). The tissue XO, MDA and MPO levels of the NEC + ginger group were significantly lower than those in the NEC group (P < 0.05).
Ginger therapy efficiently ameliorated the severity of intestinal damage in NEC and may be a promising treatment option.
坏死性小肠结肠炎（NEC）是影响早产儿的最重要的胃肠道急症，可导致严重的发病率和死亡率。但是，目前尚无治愈方法。氧化应激、炎症、细胞凋亡以及早产被认为与疾病的发病机制有关。生姜及其化合物具有抗炎、抗菌、抗氧化特性以及免疫调节、细胞保护/再生作用。
本研究旨在评估生姜对 NEC 实验大鼠模型肠道损伤的有益作用。
在这项实验研究中，将 30 只新生 Wistar 大鼠分为三组：NEC 组、NEC+生姜组和对照组。通过腹腔内注射脂多糖、肠内配方喂养、缺氧-复氧和冷应激暴露诱导 NEC。NEC+生姜组的幼鼠口服生姜，剂量为 1000mg/kg/天。切除近端结肠和回肠。进行组织病理学、免疫组织化学（TUNEL 用于凋亡、半胱天冬酶 3 和 8）和生化测定，包括黄嘌呤氧化酶（XO）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、丙二醛（MDA）和髓过氧化物酶（MPO）、肿瘤坏死因子-α（TNF-α）、白细胞介素 1β（IL-1β）和白细胞介素 6（IL-6）活性。
与 NEC 组相比，NEC+生姜组的大鼠临床疾病评分和体重增加更好（p<0.05）。宏观评估、组织病理学和凋亡评估（TUNEL、半胱天冬酶 3 和 8）显示，NEC+生姜组肠道损伤的严重程度明显降低（p<0.05）。生姜治疗组的 TNF-α、IL-1β 和 IL-6 水平显著降低（P<0.05）。生姜治疗组的 GSH-Px 和 SOD 水平明显保存（p<0.05）。NEC+生姜组的组织 XO、MDA 和 MPO 水平明显低于 NEC 组（P<0.05）。
生姜治疗可有效改善 NEC 肠道损伤的严重程度，可能是一种有前途的治疗选择。
