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分支酸合酶。5-烯醇丙酮酸莽草酸-3-磷酸脱磷酸的稳态前动力学。

Chorismate synthase. Pre-steady-state kinetics of phosphate release from 5-enolpyruvylshikimate 3-phosphate.

作者信息

Hawkes T R, Lewis T, Coggins J R, Mousdale D M, Lowe D J, Thorneley R N

机构信息

I.C.I. Agrochemical, Jcalotts Hill Research Station, Bracknell, Berks, U.K.

出版信息

Biochem J. 1990 Feb 1;265(3):899-902. doi: 10.1042/bj2650899.

DOI:10.1042/bj2650899
PMID:2407239
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1133717/
Abstract

The pre-steady-state kinetics of phosphate formation from 5-enolpyruvylshikimate 3-phosphate catalysed by Escherichia coli chorismate synthase (EC 4.6.1.4) were studied by a rapid-acid-quench technique at 25 degrees C at pH 7.5. No pre-steady-state 'burst' or 'lag' phase was observed, showing that phosphate is released concomitant with the rate-limiting step of the enzyme. The implications of this result for the mechanism of action of chorismate synthase are discussed.

摘要

采用快速酸淬灭技术，在25℃、pH 7.5条件下，研究了大肠杆菌分支酸合酶（EC 4.6.1.4）催化5-烯醇丙酮酸莽草酸-3-磷酸生成磷酸盐的前稳态动力学。未观察到前稳态“爆发”或“滞后”阶段，表明磷酸盐的释放与该酶的限速步骤同步。讨论了这一结果对分支酸合酶作用机制的影响。

相似文献

1
Chorismate synthase. Pre-steady-state kinetics of phosphate release from 5-enolpyruvylshikimate 3-phosphate.分支酸合酶。5-烯醇丙酮酸莽草酸-3-磷酸脱磷酸的稳态前动力学。
Biochem J. 1990 Feb 1;265(3):899-902. doi: 10.1042/bj2650899.
2
Escherichia coli chorismate synthase: a deuterium kinetic-isotope effect under single-turnover and steady-state conditions shows that a flavin intermediate forms before the C-(6proR)-H bond is cleaved.大肠杆菌分支酸合酶：单周转和稳态条件下的氘动力学同位素效应表明，在C-（6proR）-H键断裂之前会形成黄素中间体。
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Evidence for a major structural change in Escherichia coli chorismate synthase induced by flavin and substrate binding.黄素和底物结合诱导大肠杆菌分支酸合酶发生重大结构变化的证据。
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Studies with substrate and cofactor analogues provide evidence for a radical mechanism in the chorismate synthase reaction.对底物和辅因子类似物的研究为分支酸合酶反应中的自由基机制提供了证据。
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本文引用的文献

1
The herbicide glyphosate is a potent inhibitor of 5-enolpyruvyl-shikimic acid-3-phosphate synthase.除草剂草甘膦是5-烯醇丙酮酰莽草酸-3-磷酸合酶的强效抑制剂。
Biochem Biophys Res Commun. 1980 Jun 30;94(4):1207-12. doi: 10.1016/0006-291x(80)90547-1.
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The use of inhibitors in the study of glycosidases.糖苷酶研究中抑制剂的应用。
Biochimie. 1982 Nov-Dec;64(11-12):977-1000. doi: 10.1016/s0300-9084(82)80379-9.
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Stereochemistry of chorismic acid biosynthesis.
J Am Chem Soc. 1969 Oct 8;91(21):5893-4. doi: 10.1021/ja01049a045.
4
Stereochemistry of the 3-deoxy-D-arabino-heptulosonate 7-phosphate synthetase reaction and the chorismate synthetase reaction.3-脱氧-D-阿拉伯庚酮糖酸7-磷酸合成酶反应与分支酸合成酶反应的立体化学
J Biol Chem. 1972 Feb 10;247(3):736-44.
5
The overexpression, purification and complete amino acid sequence of chorismate synthase from Escherichia coli K12 and its comparison with the enzyme from Neurospora crassa.大肠杆菌K12分支酸合酶的过表达、纯化及完整氨基酸序列，及其与粗糙脉孢菌该酶的比较。
Biochem J. 1988 Apr 15;251(2):313-22. doi: 10.1042/bj2510313.
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The arom multifunctional enzyme from Neurospora crassa.来自粗糙脉孢菌的芳烃多功能酶。
Methods Enzymol. 1987;142:325-41. doi: 10.1016/s0076-6879(87)42044-2.
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An improved assay for nanomole amounts of inorganic phosphate.一种用于检测纳摩尔量无机磷酸盐的改进测定法。
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