Departments of Pediatrics, College of Medicine and Health Sciences, United Arab Emirates University, P.O. Box 17666, Al Ain, United Arab Emirates.
Virology. 2013 Nov;446(1-2):180-8. doi: 10.1016/j.virol.2013.07.034. Epub 2013 Aug 29.
Inhibition of cellular respiration, oxidation of glutathione and induction of apoptosis have been reported in epithelial cells infected in vitro with influenza A virus (IAV). Here, the same biomarkers were investigated in vivo by assessing the lungs of BALB/c mice infected with IAV. Cellular respiration declined on day 3 and recovered on day 7 post-infection. For days 3-5, the rate (mean±SD) of respiration (µMO2min(-1)mg(-1)) in uninfected lungs was 0.103±0.021 (n=4) and in infected lungs was 0.076±0.025 (n=4, p=0.026). Relative cellular ATP (infected/uninfected) was 4.7 on day 2 and 1.07 on day 7. Intracellular caspase activity peaked on day 7. Cellular glutathione decreased by ≥10% on days 3-7. Lung pathology was prominent on day 3 and caspase-3 labeling was prominent on day 5. IAV infection was associated with suppression of cellular respiration, diminished glutathione, and induction of apoptosis. These functional biomarkers were associated with structural changes noted in infected mice.
在体外感染甲型流感病毒 (IAV) 的上皮细胞中，已报道细胞呼吸抑制、谷胱甘肽氧化和细胞凋亡诱导。在此，通过评估感染 IAV 的 BALB/c 小鼠的肺部，对这些相同的生物标志物进行了体内研究。感染后第 3 天细胞呼吸下降，第 7 天恢复。在第 3-5 天，未感染肺部的呼吸率（µMO2min(-1)mg(-1)）平均值±标准差为 0.103±0.021（n=4），感染肺部为 0.076±0.025（n=4，p=0.026）。第 2 天细胞内 ATP 相对值（感染/未感染）为 4.7，第 7 天为 1.07。细胞内半胱氨酸天冬氨酸蛋白酶活性在第 7 天达到峰值。细胞内谷胱甘肽在第 3-7 天减少了≥10%。第 3 天肺部病理变化明显，第 5 天 caspase-3 标记明显。IAV 感染与细胞呼吸抑制、谷胱甘肽减少和细胞凋亡诱导有关。这些功能生物标志物与感染小鼠的结构变化有关。
