ETH Zurich, Institute for Chemical- and Bioengineering, Wolfgang-Pauli-Strasse 10, CH-8093 Zurich, Switzerland.
J Chromatogr A. 2013 Nov 1;1314:77-85. doi: 10.1016/j.chroma.2013.08.102. Epub 2013 Sep 8.
Chromatographic stationary phases such as Fractogel EMD SO3 (M) have a pore size distribution that is close to the size of proteins. The accessible porosity and the mass transfer inside the particles are therefore strongly affected by the pore to solute size ratio. This effect was simulated using the distributed pore model for three media: Base Fractogel SO3, Fractogel EMD SO3 (M) and (S). This model was extended so as to be able to account for the effect of pore shrinkage due to protein loading on the chromatographic behavior of other proteins. Pulse chromatographic experiments using dextrans of various sizes on column pre-loaded with antibodies have been conducted to test the model reliability.
色谱固定相，如 Fractogel EMD SO3（M），具有接近蛋白质大小的孔径分布。因此，孔到溶质大小比强烈影响可及孔隙率和颗粒内的质量传递。使用分布孔模型模拟了三种介质的这种效应：基础 Fractogel SO3、Fractogel EMD SO3（M）和（S）。该模型进行了扩展，以便能够考虑由于蛋白质负载导致的孔收缩对其他蛋白质色谱行为的影响。使用各种大小的葡聚糖在预先加载抗体的柱上进行脉冲色谱实验，以测试模型的可靠性。
