Department of Clinical Laboratory, Shandong Provincial Hospital Affiliated to Shandong University.
Department of Clinical Laboratory, Shandong Provincial Hospital Affiliated to Shandong University.
J Diabetes Complications. 2014 Jan-Feb;28(1):96-100. doi: 10.1016/j.jdiacomp.2013.08.006. Epub 2013 Sep 24.
We tested whether urinary podocalyxin-positive element (PCX+EL) can be a marker of early stage of diabetic nephropathy (DN).
DN patients (n=68) and health controls (n=28) were enrolled in this study. Patients were classified into three groups: normoalbuminuria, microalbuminuria and macroalbuminuria. Urinary PCX+EL, serum cystatin C (Scys C) and serum creatinine (SCr) were quantified, and correlations between urinary PCX+EL and urinary albumin, Scys C and SCr were examined. The comparison of diagnosis efficiency among urinary PCX+EL, Scys C and SCr was made by receiver operating characteristic (ROC) analysis.
Urinary PCX+EL, Scys C and SCr significantly increased in DN patients compared with controls. Urinary PCX+EL increased significantly in all three patients groups compared with controls. However, the concentration of Scys C and SCr did not increase in normoalbuminuria group. Urinary albumin, Scys C and SCr correlated with urinary PCX+EL. ROC curve analysis indicated that area under the curve (AUC) of urinary PCX+EL (0.966) is higher than that of Scys C (0.857) and SCr (0.726) for discriminating nephropathy between DN patients and controls.
Urinary PCX+EL may be a noninvasive marker for the early stage of DN.
本研究旨在探讨尿足细胞标志蛋白阳性物（PCX+EL）能否成为糖尿病肾病（DN）早期阶段的标志物。
本研究纳入了 68 例 DN 患者和 28 例健康对照者。患者被分为三组：正常白蛋白尿组、微量白蛋白尿组和大量白蛋白尿组。定量检测尿 PCX+EL、血清胱抑素 C（Scys C）和血清肌酐（SCr），并分析尿 PCX+EL 与尿白蛋白、Scys C 和 SCr 之间的相关性。通过受试者工作特征（ROC）曲线分析比较尿 PCX+EL、Scys C 和 SCr 的诊断效率。
与对照组相比，DN 患者的尿 PCX+EL、Scys C 和 SCr 显著升高。与对照组相比，所有三组患者的尿 PCX+EL 均显著升高。然而，在正常白蛋白尿组中，Scys C 和 SCr 的浓度并未升高。尿白蛋白、Scys C 和 SCr 与尿 PCX+EL 相关。ROC 曲线分析表明，尿 PCX+EL 的曲线下面积（AUC）（0.966）高于 Scys C（0.857）和 SCr（0.726），用于区分 DN 患者和对照组的肾病。
尿 PCX+EL 可能是早期 DN 的一种非侵入性标志物。
