Postel-Vinay M C, Durand D, López S, Kayser C, Lavau M
INSERM Unité 30, Hôpital des Enfants-Malades, Paris, France.
Horm Metab Res. 1990 Jan;22(1):7-11. doi: 10.1055/s-2007-1004837.
Developmental changes in hepatic growth hormone binding sites were examined in the genetically obese male fa/fa rats and in the lean littermates. At 16 days, fa/fa pups are normoinsulinemic; the specific binding of 125I-hGH to liver membranes is comparable in the two genotypes. At 4 weeks and later on, plasma membranes and Golgi fractions of male obese Zucker rats have more GH binding sites than lean littermates. The GH pituitary content is comparable in the two genotypes from 2 to 8 weeks and in 14-week-old fa/fa rats it is half that in lean animals. In the two genotypes plasma IGFI dramatically increases during puberty. At 4 weeks, plasma IGFI level is significantly higher in fa/fa rats than in lean littermates. In this model of genetic obesity, an increased GH binding to liver membranes is observed after the third week of life, shortly after the onset of hyperinsulinemia in the fa/fa rat.
在遗传性肥胖雄性fa/fa大鼠及其瘦的同窝仔鼠中研究了肝脏生长激素结合位点的发育变化。在16日龄时，fa/fa幼崽正常胰岛素血症；两种基因型中125I-hGH与肝细胞膜的特异性结合相当。在4周及之后，雄性肥胖 Zucker 大鼠的质膜和高尔基体部分比瘦的同窝仔鼠有更多的生长激素结合位点。在2至8周龄时，两种基因型的垂体生长激素含量相当，而在14周龄的fa/fa大鼠中，其含量是瘦动物的一半。在两种基因型中，青春期期间血浆胰岛素样生长因子I（IGFI）显著增加。在4周龄时，fa/fa大鼠的血浆IGFI水平显著高于瘦的同窝仔鼠。在这种遗传性肥胖模型中，在出生后第三周，即fa/fa大鼠高胰岛素血症开始后不久，观察到生长激素与肝细胞膜的结合增加。
