Jiangsu Key Laboratory for High Technology Research of TCM Formulae, Nanjing University of Chinese Medicine, 138 Xianlin Road, Nanjing 210023, China.
J Pharm Biomed Anal. 2014 Jan;88:207-15. doi: 10.1016/j.jpba.2013.08.043. Epub 2013 Sep 7.
Astragali Radix (AR) and Angelicae Sinensis Radix (ASR) were frequently combined and used in China as herbal pair called as Danggui Buxue Decoction (DBD) for treatment of blood deficiency syndrome, such as women's ailments. This study is to investigate the tissue distribution profiles of five major bio-active constituents (ferulic acid, caffeic acid, calycosin-7-O-β-glucoside, ononin and astragaloside IV) in DBD after oral administration of DBD in blood deficiency rats, and to compare the difference between normal and blood deficiency rats. The blood deficiency rats were induced by bleeding from orbit at the dosages of 5.0mLkg(-1) every day, and the experimental period was 12 days. At the finally day of experimental period, both normal and blood deficiency rats were orally administrated with DBD, and then the tissues samples were collected at different time points. Ferulic acid, caffeic acid, calycosin-7-O-β-glucoside, ononin and astragaloside IV in different tissues were detected simultaneously by UPLC-TQ/MS, and the histograms were drawn. The results showed that the overall trend was CLiver>CKidney>CHeart>CSpleen>CLung, CC-30min>CM-30min>CM-60min>CC-5min>CM-5min>CC-60min>CM-240min>CC-240min. The contents of the detected compounds in liver were more than that in other tissues no matter in normal or blood deficiency rats. Compared to normal rats, partial contents of the compounds in blood deficiency rats' tissues at different time points had significant difference (P<0.05). This study was the first report about tissue distribution investigation in blood deficiency animals which is conducted by bleeding. And the results demonstrated that the five DBD components in normal and blood deficiency rats had obvious differences in some organs and time points, suggesting that the blood flow and perfusion rate of the organ were altered in blood deficiency animals.
黄芪（AR）和当归（ASR）经常合并使用，在中国作为草药对（DBD）用于治疗血虚症，如妇女疾病。本研究旨在研究口服血虚大鼠 DBD 后，五种主要生物活性成分（阿魏酸、咖啡酸、毛蕊异黄酮-7-O-β-葡萄糖苷、芒柄花苷和黄芪甲苷）在 DBD 中的组织分布特征，并比较正常和血虚大鼠之间的差异。血虚大鼠通过每天眼眶采血 5.0mLkg(-1) 诱导，实验期为 12 天。在实验期的最后一天，正常和血虚大鼠均口服 DBD，然后在不同时间点采集组织样本。采用 UPLC-TQ/MS 同时检测不同组织中的阿魏酸、咖啡酸、毛蕊异黄酮-7-O-β-葡萄糖苷、芒柄花苷和黄芪甲苷，并绘制图谱。结果表明，总体趋势为 CLiver>CKidney>CHeart>CSpleen>CLung，CC-30min>CM-30min>CM-60min>CC-5min>CM-5min>CC-60min>CM-240min>CC-240min。无论在正常还是血虚大鼠中，肝脏中检测到的化合物含量均高于其他组织。与正常大鼠相比，血虚大鼠各时间点部分组织中化合物含量存在显著差异（P<0.05）。本研究是首次通过采血对血虚动物进行组织分布研究的报道。结果表明，正常和血虚大鼠 DBD 中的五种成分在某些器官和时间点存在明显差异，提示血虚动物器官血流和灌注率发生改变。
