Screening study of blood-supplementing active components in water decoction of processed with yellow rice wine based on response surface methodology.

CONTEXT

(Oliv.) Diels (Apiaceae) (syn. Maxim var. Oliver) processed with yellow rice wine (WAS) has a blood-supplementing effect.

OBJECTIVE

To establish an optimal technology for preparing water decoction of WAS (WASD), and screen blood-supplementing fractions.

MATERIALS AND METHODS

Ferulic acid and crude polysaccharide were used in optimizing the preparation technology for WASD through response surface methodology. The independent variables were liquid-solid ratio, soaking time, and extraction time. Eighty Kunming mice were randomly divided into normal control, model, and six intervention groups ( = 10). The intervention groups were given different WASD fractions by gavage (5 or 10 g/kg). The model intervention groups received acetylphenyl hydrazine (subcutaneous injection) and cyclophosphamide (intraperitoneal injection). Duration of study, 9 days. The components of blood-supplementing fractions were analyzed.

RESULTS

The optimum extraction parameters were liquid-solid ratio, 7.69:1 mL/g; soaking time, 119.78 min; and extraction time, 143.35 min. The optimal OD value was 0.8437. RBC, WBC, and Hb in the water fraction (5, 10 g/kg) and -butanol fraction (10 g/kg) intervention groups increased significantly compared with the model group ( < 0.05). Polysaccharide and caffeic acid contents of water fraction were 252.565 and 0.346 μg/mg, respectively; ferulic acid was not detected. Caffeic acid and ferulic acid contents of -butanol fraction were 1.187 and 0.806 μg/mg, respectively, polysaccharide was not detected.

CONCLUSIONS

The optimum preparation technology of WASD was obtained, and the water, -butanol fractions were blood-supplementing fractions. This study provides a theoretical foundation for further application of WAS in the pharmaceutical industry.