Alyasin Soheila, Karimi Mohammad Hossein, Amin Reza, Babaei Maryam, Darougar Sepideh
Allergy Research Center, Shiraz University of Medical Sciences, Shiraz, Iran, e-mail:
Iran J Immunol. 2013 Sep;10(3):177-85.
IL-17 is a major cytokine player in T cell mediated leukocyte associated inflammation. IL-17 is also recognized to participate in the pathophysiology of asthma.
To determine the role of IL-17 in predicting severe asthma.
We obtained serum samples from asthmatic children under the age of 5-year in three different groups of mild (n=33), moderate (n=28) and severe (n=32) persistent asthma. IL-17 serum concentrations and mRNA expression were determined by ELISA and real time PCR assays, respectively.
Serum IL-17 concentrations were significantly higher in patients with severe asthma than the other two groups of children with mild and moderate disease (p=0.00). Mean serum IL-17 values were 142.04 pg/ml in mild group, 180.4 pg/ml in moderate group and 251.25 pg/ml in severe group. IL-17 mRNA levels were also significantly elevated in severe asthmatic patients compared to mild and moderate asthmatic children (p=0.00).
Our data reveal an increase in the serum IL-17 concentration and IL-17 mRNA expression in children with severe asthma compared to those with mild and moderate forms of the disease.
白细胞介素-17（IL-17）是T细胞介导的白细胞相关炎症中的主要细胞因子。IL-17也被认为参与哮喘的病理生理学过程。
确定IL-17在预测重度哮喘中的作用。
我们从三组不同的5岁以下哮喘儿童中获取血清样本，分别为轻度持续性哮喘组（n = 33）、中度持续性哮喘组（n = 28）和重度持续性哮喘组（n = 32）。分别通过酶联免疫吸附测定（ELISA）和实时聚合酶链反应（PCR）检测血清IL-17浓度和mRNA表达。
重度哮喘患者的血清IL-17浓度显著高于轻度和中度哮喘的另外两组儿童（p = 0.00）。轻度组血清IL-17平均水平为142.04 pg/ml，中度组为180.4 pg/ml，重度组为251.25 pg/ml。与轻度和中度哮喘儿童相比，重度哮喘患者的IL-17 mRNA水平也显著升高（p = 0.00）。
我们的数据显示，与轻度和中度哮喘患儿相比，重度哮喘患儿的血清IL-17浓度和IL-17 mRNA表达增加。
