大鼠肾脏中犬尿氨酸丙酮酸转氨酶的亚细胞分布及特性

Subcellular distribution and properties of kynurenine pyruvate transaminase in rat kidney.

作者信息

Noguchi T, Nakatani M, Minatogawa Y, Morimoto M, Kido R

出版信息

Hoppe Seylers Z Physiol Chem. 1975 Aug;356(8):1245-50. doi: 10.1515/bchm2.1975.356.2.1245.

DOI:10.1515/bchm2.1975.356.2.1245

Abstract

Kynurenine transaminase activity in rat kidney was found in both the mitochondrial and supernatant fractions. These fractions contained (a) kynurenine pyruvate transaminase, which showed a preference for pyruvate as amino acceptor, and had a pH optimum between 8.0 and 8.5, and (b) kynurenine 2-oxoglutarate transaminase, with a preference for 2-oxoglutarate and a pH optimum between 6.0 and 6.5. The apparent Km value of the former enzyme for L-kynurenine was much lower than that of the latter enzyme.

摘要

在大鼠肾脏中，犬尿氨酸转氨酶活性存在于线粒体和上清液组分中。这些组分含有：(a) 犬尿氨酸丙酮酸转氨酶，它优先选择丙酮酸作为氨基受体，最适pH在8.0至8.5之间；以及(b) 犬尿氨酸2-氧代戊二酸转氨酶，优先选择2-氧代戊二酸，最适pH在6.0至6.5之间。前一种酶对L-犬尿氨酸的表观Km值远低于后一种酶。