Amino Acids. 2013 Dec;45(6):1431-42. doi: 10.1007/s00726-013-1596-8.
Combinatorial peptide ligand libraries have been extensively used for the enrichment of very low-abundance proteins, while concomitantly reducing the concentration of major species. A number of biological extracts have been reported with great success. Nevertheless, there are examples where the enrichment was not as good as expected. It has been demonstrated that the protocol itself may be responsible for the final results and is not necessarily applied as it should. In this paper, technical details along with important suggestions are given for an optimized enrichment of gene products present at trace levels among very many other proteins.
组合肽配体文库已被广泛用于富集极低丰度的蛋白质，同时降低主要物质的浓度。已经有许多生物提取物被成功报道。然而，也有一些例子表明，富集效果并不如预期的那样好。已经证明，方案本身可能是导致最终结果的原因，而且并不一定按照应有的方式实施。在本文中，提供了技术细节以及重要建议，以优化在非常多其他蛋白质中痕量存在的基因产物的富集。
