一种新型α-葡萄糖苷酶抑制剂对Zucker肥胖大鼠脂肪肝的抑制作用

Inhibitory effect of a new alpha-glucosidase inhibitor on fatty liver in Zucker fatty rats.

作者信息

Inui Y, Kawata S, Matsuzawa Y, Tokunaga K, Fujioka S, Tamura S, Kobatake T, Keno Y, Odaka H, Matsuo T

机构信息

Second Department of Internal Medicine, Osaka University Medical School, Japan.

出版信息

J Hepatol. 1990 Jan;10(1):62-8. doi: 10.1016/0168-8278(90)90074-2.

DOI:10.1016/0168-8278(90)90074-2

PMID:2407772

Abstract

The livers of Zucker fatty (fa/fa) and lean (Fa/-) rats treated with standard rat chow diet containing 0, 10 or 50 ppm alpha-glucosidase inhibitor (AO-128) for 10 weeks were studied morphologically and biochemically. Light microscopic examination of livers from untreated Zucker fatty rats showed severe steatosis. The triglyceride content in the livers from Zucker fatty rats was significantly higher than that from lean rats (73 +/- 9 micrograms/mg protein for Zucker fatty rats vs. 30 +/- 10 for lean rats, p less than 0.01). Administration of the inhibitor caused a marked decrease in the number and size of lipid droplets in the hepatocytes from Zucker fatty rats and a decrease in the triglyceride content in the liver (73 +/- 9 micrograms/mg protein for untreated, 54 +/- 16 for 10-ppm-treated and 48 +/- 23 for 50-ppm-treated rats, p less than 0.05). This is the first report showing an inhibitory effect of an alpha-glucosidase inhibitor on steatosis in Zucker fatty rats.

摘要

对喂食含0、10或50 ppmα-葡萄糖苷酶抑制剂（AO - 128）的标准大鼠饲料10周的Zucker肥胖（fa/fa）和瘦型（Fa/-）大鼠的肝脏进行了形态学和生物化学研究。对未处理的Zucker肥胖大鼠肝脏进行光镜检查显示有严重的脂肪变性。Zucker肥胖大鼠肝脏中的甘油三酯含量显著高于瘦型大鼠（Zucker肥胖大鼠为73±9微克/毫克蛋白质，瘦型大鼠为30±10，p＜0.01）。给予该抑制剂导致Zucker肥胖大鼠肝细胞中脂滴数量和大小显著减少，肝脏中甘油三酯含量降低（未处理大鼠为73±9微克/毫克蛋白质，10 ppm处理组为54±16，50 ppm处理组为48±23，p＜0.05）。这是首次报道α-葡萄糖苷酶抑制剂对Zucker肥胖大鼠脂肪变性有抑制作用。