Abravaya K, Rothman-Denes L B
Department of Molecular Genetics, University of Chicago, IL 60637.
J Mol Biol. 1990 Jan 20;211(2):359-72. doi: 10.1016/0022-2836(90)90357-R.
The RNA polymerase responsible for the synthesis of coliphage N4 middle RNAs, N4 RNA polymerase II, is composed of two subunits of 30,000 and 40,000 molecular weight. It is the smallest DNA-dependent RNA polymerase characterized to date. We have determined the sequences surrounding the sites of in vivo transcription initiation for this enzyme. Two regions of sequence homology are present: a box at +1, 3' AAAT 5', and a box, 3' TTCTGGAC 5' at a variable distance (16 to 24 base-pairs) upstream from +1. Possible mechanisms for recognition of these sequences are discussed.
负责合成大肠杆菌噬菌体N4中期RNA的RNA聚合酶，即N4 RNA聚合酶II，由分子量为30,000和40,000的两个亚基组成。它是迄今为止所鉴定出的最小的依赖DNA的RNA聚合酶。我们已经确定了该酶在体内转录起始位点周围的序列。存在两个序列同源区域：位于转录起始位点下游+1处的一个框，序列为3' AAAT 5'，以及一个位于+1上游可变距离（16至24个碱基对）处的框，序列为3' TTCTGGAC 5'。文中讨论了识别这些序列的可能机制。
