Chandler Christopher M, Habig Jeffrey W, Fisher Ashley A, Ambrose Katherine V, Jiménez Susana T, McDougal Owen M
Department of Chemistry and Biochemistry, Boise State University, Boise, ID 83725-1520, USA.
Nat Prod Commun. 2013 Aug;8(8):1059-64.
Four steroidal alkaloids extracted from the roots and rhizomes of Veratrum californicum were separated by high performance liquid chromatography (HPLC) and identified using high resolution electrospray ionization time of flight tandem mass spectrometry (ESI-TOF-MS/MS) as veratrosine, cycloposine, veratramine, and cyclopamine. This paper compares ethanol and benzene as extraction solvents, HPLC solvent conditions leading to good separation of steroidal alkaloids, and MS/MS fragmentation patterns for the four steroidal alkaloids which have been released to the public database MassBank.jp. The reported Soxhlet extraction method nearly triples the recovery of steroidal alkaloids from V. californicum.
从加州藜芦的根和根茎中提取的四种甾体生物碱，通过高效液相色谱（HPLC）进行分离，并使用高分辨率电喷雾电离飞行时间串联质谱（ESI-TOF-MS/MS）鉴定为藜芦胺、环泊生、藜芦胺碱和环杷明。本文比较了乙醇和苯作为提取溶剂、能使甾体生物碱良好分离的HPLC溶剂条件，以及已发布到公共数据库MassBank.jp的这四种甾体生物碱的MS/MS裂解模式。报道的索氏提取法使从加州藜芦中提取甾体生物碱的回收率提高了近两倍。
