Department of Medicine, University of Toronto School of Medicine, Toronto, Ontario, Canada; Mediprobe Research, Inc., London, Ontario, Canada.
Clin Dermatol. 2013 Sep-Oct;31(5):540-3. doi: 10.1016/j.clindermatol.2013.06.009.
The diagnosis of onychomycosis may require the use of mycological diagnostic testing to supplement the clinical presentation. In order to verify that onychomycosis is present, the viability, penetrance, and species of the fungal infection should be determined. The most common diagnostic tests are mycological culture, direct microscopic examination, histopathologic study, and polymerase chain reaction (PCR)-based tests. Each of these tests has individual strengths in satisfying the three criteria, with a minimum of two positive diagnostic tests being the gold standard for confirming all three diagnostic criteria.
甲真菌病的诊断可能需要使用真菌学诊断检测来补充临床表现。为了验证是否存在甲真菌病，应确定真菌感染的活力、穿透力和种类。最常见的诊断检测包括真菌培养、直接显微镜检查、组织病理学研究和基于聚合酶链反应（PCR）的检测。这些检测方法各有优势，可分别满足三个标准中的一个，至少有两个阳性诊断检测可作为同时满足所有三个诊断标准的金标准。
