Ueda Shuji, Tu-Sekine Becky, Yamanoue Minoru, Raben Daniel M, Shirai Yasuhito
Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo, Japan.
BMC Dev Biol. 2013 Oct 1;13:35. doi: 10.1186/1471-213X-13-35.
Diacylglycerol kinase (DGK) is a key enzyme that regulates diacylglycerol (DG) turnover and is involved in a variety of physiological functions. The isoform DGKθ has a unique domain structure and is the sole member of type V DGK. To reveal the spatial and temporal expression of DGKθ we performed immunohistochemical staining on paraffin sections of mouse embryos.
At an early stage of development (E10.5 and 11.5), the expression of DGKθ was prominently detected in the brain, spinal cord, dorsal root ganglion, and limb bud, and was also moderately detected in the bulbus cordis and the primordium of the liver and gut. At later stages (E12.5 and 14.5), DGKθ expression persisted or increased in the neocortex, epithalamus, hypothalamus, medulla oblongata, and pons. DGKθ was also evident in the epidermis, and nearly all epithelia of the oropharyngeal membrane, digestive tract, and bronchea. At prenatal developmental stages (E16.5 and E18.5), the expression pattern of DGKθ was maintained in the central nervous system, intestine, and kidney, but was attenuated in the differentiated epidermis.
These results suggest that DGKθ may play important physiological roles not only in the brain, but also in diverse organs and tissues during the embryonic stages.
二酰基甘油激酶（DGK）是一种调节二酰基甘油（DG）周转的关键酶，参与多种生理功能。异构体DGKθ具有独特的结构域结构，是V型DGK的唯一成员。为了揭示DGKθ的时空表达，我们对小鼠胚胎石蜡切片进行了免疫组织化学染色。
在发育早期（E10.5和11.5），在脑、脊髓、背根神经节和肢芽中显著检测到DGKθ的表达，在心脏球、肝脏和肠道原基中也中度检测到。在后期（E12.5和14.5），DGKθ在新皮层、上丘脑、下丘脑、延髓和脑桥中的表达持续或增加。DGKθ在表皮以及口咽膜、消化道和支气管的几乎所有上皮中也很明显。在产前发育阶段（E16.5和E18.5），DGKθ的表达模式在中枢神经系统、肠道和肾脏中保持，但在分化的表皮中减弱。
这些结果表明，DGKθ不仅可能在大脑中，而且在胚胎阶段的各种器官和组织中发挥重要的生理作用。
