Division of Pharmacology, School of Pharmacy, International University of Health and Welfare, Ohtawara, Japan; Department of Molecular Immunology, Institute of DNA medicine, Jikei University, Tokyo, Japan.
Exp Dermatol. 2013 Oct;22(10):669-71. doi: 10.1111/exd.12222.
We investigated the effects of a single dose of mouse interleukin-31 (IL-31) on scratching behaviour in comparison with spontaneous skin-lesion- or serotonin (5-HT)- induced scratching behaviour in NC/Nga and BALB/c mice. Intradermal (i.d.) injection of IL-31 caused a gradual increase in long-lasting scratching (LLS, over 1.5 s) about 3 h after administration followed by a gradual decrease for over 24 h after administration. I.d. injection of IL-31 significantly increased the total LLS counts/24 h but not short-lasting scratching (SLS, 0.3-1.5 s). In skin-lesioned NC/Nga mice, the LLS but not SLS counts were significantly higher than those in non-skin-lesioned NC/Nga mice. We also investigated 5-HT-induced scratching in BALB/c mice, SLS but not LLS increased immediately after the injection and then decreased to baseline after at 20 min. These results suggest that IL-31 may participate in the sensation of itching and promote scratching behaviour in skin-lesioned NC/Nga mice, an animal model of atopic dermatitis (AD).
我们研究了单次给予小鼠白细胞介素-31（IL-31）对搔抓行为的影响，并与自发性皮肤损伤或 5-羟色胺（5-HT）诱导的搔抓行为进行了比较，在 NC/Nga 和 BALB/c 小鼠中进行了研究。IL-31 皮内（i.d.）注射后约 3 小时引起持续时间较长的搔抓（LLS，超过 1.5 秒）逐渐增加，随后在给药后 24 小时逐渐减少。IL-31 的 i.d. 注射显著增加了总 LLS 计数/24 小时，但不增加短暂搔抓（SLS，0.3-1.5 秒）。在皮肤损伤的 NC/Nga 小鼠中，LLS 计数而非 SLS 计数明显高于非皮肤损伤的 NC/Nga 小鼠。我们还研究了 BALB/c 小鼠中 5-HT 诱导的搔抓行为，SLS 但不是 LLS 在注射后立即增加，然后在 20 分钟后降至基线。这些结果表明，IL-31 可能参与瘙痒感，并促进特应性皮炎（AD）动物模型 NC/Nga 小鼠的搔抓行为。
