Aix-Marseille Université, Institut de Chimie Radicalaire ICR - UMR CNRS 7273, équipe CMO, case 562, avenue Escadrille Normandie-Niemen, 13397 Marseille Cedex 20, France.
Colloids Surf B Biointerfaces. 2013 Dec 1;112:513-20. doi: 10.1016/j.colsurfb.2013.07.057. Epub 2013 Sep 10.
Two novel enediynes containing an aromatic ring and substituted by two thiol functions as end-groups were designed and studied as functionalizing agent of gold nanoparticles. Phototriggered cyclization of the capping agent under UV-visible irradiation was investigated. Interestingly, the length of the thiol-substituted chain was shown to influence significantly the cyclization rate. Depending on the length of the spacer, either polymerization or simple cyclization of the coating agent was evidenced. The present study underscores the possibility of finely controlling the fate of the coating agent (polymerization/cyclization). Nanocomposites were characterized by UV-visible absorption spectroscopy, dynamic light scattering (DLS) technique and transmission electron microscopy (TEM) measurements. Finally, the ability of the colloidal solutions to induce photoinitiated damages to PcDNA3 supercoiled DNA was evaluated. Interestingly, an increase as high as 50% of the DNA cleavage could be registered when adding enediynes-capped gold nanoparticles to solutions of enediynes. In particular, the enhancement of DNA scission was observed in both thermal and photochemical activation modes.
设计并研究了两种含有芳香环和两个巯基作为端基的新型烯二炔，将其作为金纳米粒子的功能化试剂。研究了在紫外可见辐射下，封端剂的光触发环化反应。有趣的是，硫醇取代链的长度对环化速率有显著影响。根据间隔物的长度，可以证明涂层剂发生聚合或简单环化。本研究强调了精细控制涂层剂命运（聚合/环化）的可能性。通过紫外-可见吸收光谱、动态光散射（DLS）技术和透射电子显微镜（TEM）测量对纳米复合材料进行了表征。最后，评估了胶体溶液诱导 PcDNA3 超螺旋 DNA 光引发损伤的能力。有趣的是，当向烯二炔溶液中添加烯二炔封端的金纳米粒子时，可记录到高达 50%的 DNA 断裂增加。特别是，在热和光化学激活模式下都观察到 DNA 断裂的增强。
