Department of Pesticide Science, College of Plant Protection, Nanjing Agricultural University, Jiangsu Key Laboratory of Pesticide Science, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, PR China.
Chemosphere. 2014 Jan;95:241-6. doi: 10.1016/j.chemosphere.2013.08.084. Epub 2013 Sep 27.
The enantioselective degradation behaviors of the acylamino acid fungicide metalaxyl in vegetables (cucumber, cabbage, spinach and pakchoi) under field and greenhouse conditions were elucidated by reversed-phase high-performance liquid chromatography (HPLC). A cellulose tris (3-chloro-4-methyl phenyl carbamate) chiral column (Lux Cellulose-2) was used for the chiral separation of the enantiomers using acetonitrile-0.1% formic acid solution (40:60, v/v) as mobile phase at a flow rate of 1.0 mL min(-1). The two enantiomers could be completely separated with a resolution (Rs) of 5.2. The linearity range, matrix effect, precision and recovery were evaluated. The method was successfully applied for the enantioselective analysis of metalaxyl in cucumber, cabbage, spinach and pakchoi under different conditions. The results showed that R-enantiomer dissipated faster than S-enantiomer in cucumber of greenhouse. Inversely, a preferential degradation of S-form was found in spinach and pakchoi under field and greenhouse conditions. No stereoselective degradation in cabbage was observed. These results can be applied for evaluating the environmental risk and food safety.
采用反相高效液相色谱法（HPLC）阐明了酰氨基类农药金属axyl 在田间和温室条件下蔬菜（黄瓜、白菜、菠菜和小白菜）中的对映体选择性降解行为。使用纤维素三（3-氯-4-甲基苯基氨基甲酸酯）手性柱（Lux Cellulose-2），以乙腈-0.1%甲酸溶液（40:60，v/v）为流动相，流速为 1.0 mL min(-1)，对手性对映体进行了分离。两种对映体可以完全分离，分辨率（Rs）为 5.2。评估了线性范围、基质效应、精密度和回收率。该方法成功地应用于不同条件下黄瓜、白菜、菠菜和小白菜中金属axyl 的对映体分析。结果表明，在温室黄瓜中，R-对映体的降解速度快于 S-对映体。相反，在田间和温室条件下，菠菜和小白菜中 S-形式优先降解。在白菜中未观察到立体选择性降解。这些结果可用于评估环境风险和食品安全。
