Fakhrjou Ashraf, Dastranj-Tabrizi Ali, Ghojazadeh Morteza, Ghorashi Sona, Velayati Atefeh, Piri Reza, Vahedi Amir, Sayyah-Melli Manizhe, Smaeili Heydar-Ali, Bonakdari Amir, Halimi Monireh, Naghavi-Behzad Mohammad
Department of Pathology, Tabriz University of Medical Sciences, Tabriz, Iran E-mail :
Asian Pac J Cancer Prev. 2013;14(8):4815-8. doi: 10.7314/apjcp.2013.14.8.4815.
Atrophic epithelium of cervix sampled from postmenopausal women may mimic high-grade cervical intraepithelial neoplasia in Papanicolaou-stained (Pap) smears. Ki-67 (MIB-1) protein presents on proliferating cells, and percentage of cells with positive nuclei provides a reliable tool for rapid evaluation of the growth fraction. The aim of this study was to determine the diagnostic value of protein Ki67 staining in atypical pap smears of postmenopausal women.
In a case-control setting, pap smears of 75 women with an atypical pap smear (case group) and 75 with normal pap smears (controls) were obtained before and after estrogen treatment. Afterward, samples were exposed to the monoclonal antibody Ki-67 (MIB-1) and the immunohistochemically demonstrated Ki-67+ cells were compared.
Mean ages of cases and controls were 60.4±4.5 and 59.9±4.3 years respectively (P=0.50). There was one (2.7%) positive Ki-67 specimen in the case group, without any positive Ki-67 specimen in the control group (P=0.50).
Measurement of proliferative activity index in Pap smears restrained with MIB1 is a simple, reliable, and cost-effective method for excluding negatives. This would imply that it might allow a substantial reduction of diagnostic estrogen courses and subsequent Pap smears in postmenopausal women with atypical findings.
绝经后女性宫颈萎缩性上皮在巴氏染色涂片中可能类似高级别宫颈上皮内瘤变。Ki-67（MIB-1）蛋白存在于增殖细胞中，细胞核阳性细胞的百分比为快速评估生长分数提供了一个可靠工具。本研究的目的是确定Ki67蛋白染色在绝经后女性非典型巴氏涂片中的诊断价值。
在病例对照研究中，收集75例非典型巴氏涂片女性（病例组）和75例正常巴氏涂片女性（对照组）在雌激素治疗前后的巴氏涂片。之后，样本与单克隆抗体Ki-67（MIB-1）接触，并比较免疫组织化学显示的Ki-67+细胞。
病例组和对照组的平均年龄分别为60.4±4.5岁和59.9±4.3岁（P=0.50）。病例组有1例（2.7%）Ki-67标本呈阳性，对照组无Ki-67标本呈阳性（P=0.50）。
用MIB1抑制的巴氏涂片中增殖活性指数的测定是一种简单、可靠且经济有效的排除阴性结果的方法。这意味着它可能会大幅减少绝经后有非典型发现女性的诊断性雌激素疗程及后续巴氏涂片检查。
