Chekhun S, Bezdenezhnykh N, Shvets J, Lukianova N
R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv 03022, Ukraine.
Exp Oncol. 2013 Sep;35(3):174-9.
The aim of our study was to determine the complex of molecular genetic markers which are associated with cancer aggressiveness, invasion and metastasis among different molecular subtypes of breast cancer cell lines.
The cell lines used in the analysis include T47D, MCF-7, MDA-MB-231, MDA-MB-468, MCF-10A and 184A1. Expression of estrogen receptor, progesterone receptor, Her-2/neu and Ki-67 was studied by immunocytochemical method. CD24, CD44 and E-cadherin expression was studied by flow cytometry.
We have identified biomarkers which characterize metastatic potential of human breast cancer cells of certain molecular subtypes. It has been demonstrated that low colony forming activity of human breast cancer cells of luminal subtype is accompanied by increased adhesive properties of these cells due to high level of E-cadherin expression, low level of CD44 expression and absence of CD24 expression. High tumorigenicity of cells of basal subtype is connected to weakening of adhesive contacts that is caused by abnormalities of E-cadherin expression, significant increase of CD44 expression and presence of low level of CD24 expression.
Our data indicated that changes of correlation between expression of cellular adhesion molecules inside conventional immunohistochemical subtypes reflect significantly wider biological properties of luminal and basal subtypes of human breast cancer.
我们研究的目的是确定与乳腺癌细胞系不同分子亚型中的癌症侵袭性、浸润和转移相关的分子遗传标志物复合物。
分析中使用的细胞系包括T47D、MCF-7、MDA-MB-231、MDA-MB-468、MCF-10A和184A1。采用免疫细胞化学方法研究雌激素受体、孕激素受体、Her-2/neu和Ki-67的表达。通过流式细胞术研究CD24、CD44和E-钙黏蛋白的表达。
我们已经鉴定出表征某些分子亚型人乳腺癌细胞转移潜能的生物标志物。已经证明，管腔亚型人乳腺癌细胞的低集落形成活性伴随着这些细胞黏附特性的增加，这是由于E-钙黏蛋白表达水平高、CD44表达水平低以及CD24表达缺失所致。基底亚型细胞的高致瘤性与黏附接触的减弱有关，这是由E-钙黏蛋白表达异常、CD44表达显著增加以及低水平CD24表达的存在引起的。
我们的数据表明，传统免疫组织化学亚型内细胞黏附分子表达之间相关性的变化显著反映了人乳腺癌管腔和基底亚型更广泛的生物学特性。
