Malling H J, Djurup R, Stahl Skov P, Permin H, Magnusson C G, Søborg M, Søndergaard I, Weeke B
Allergy. 1985 May;40(4):250-6. doi: 10.1111/j.1398-9995.1985.tb00229.x.
A case of monoclonal IgE type lambda with IgE levels about 1 mg/ml has been followed for 6 years. Except for a slight asthma no signs of malignancies, parasitic infestations or other known diseases compatible with hyper-IgE have been found. By the combination of fractional ammonium sulphate precipitation, gel filtration, chromatofocusing, and subtraction affinity chromatography the IgE protein was isolated in an immunochemically pure and homogeneous form. Immunofluorescence of bone marrow cells showed about 1% IgE plasma cells. The amount of basophil bound IgE was 42 ng/10(6) cells, and histamine release from basophils challenged with anti-IgE was not different from that in atopic control persons, indicating a within-allergic-patients normal amount of IgE receptors. The protein-A reactivity was 0.4% equivalent to well-known IgE myeloma proteins. No antigen specificity of the IgE protein was found. Only a few cases of asymptomatic hyper-IgE are known, and it cannot be ruled out that this represents a premyeloma condition, since a similar case terminated in a malignant lymphoma.
一例单克隆IgE λ型患者，IgE水平约为1mg/ml，已随访6年。除轻度哮喘外，未发现恶性肿瘤、寄生虫感染或其他与高IgE血症相符的已知疾病迹象。通过硫酸铵分级沉淀、凝胶过滤、层析聚焦和减法亲和层析相结合的方法，以免疫化学纯且均一的形式分离出了IgE蛋白。骨髓细胞免疫荧光显示约1%的IgE浆细胞。嗜碱性粒细胞结合的IgE量为42ng/10⁶细胞，用抗IgE刺激嗜碱性粒细胞后组胺释放与特应性对照人群无异，表明在过敏患者中IgE受体数量正常。该蛋白A反应性为0.4%，与已知的IgE骨髓瘤蛋白相当。未发现IgE蛋白的抗原特异性。已知仅有少数无症状高IgE血症病例，且不能排除这代表骨髓瘤前期状态，因为有一例类似病例最终发展为恶性淋巴瘤。
