不饱和长链脂肪酸增强钙离子跨脂质体膜的转运。

Ca2+ translocation across liposomal membranes enhanced by unsaturated long-chain fatty acids.

作者信息

Utsumi K, Nobori K, Miyahara M

出版信息

Cell Struct Funct. 1985 Jun;10(2):177-81. doi: 10.1247/csf.10.177.

DOI:10.1247/csf.10.177

Abstract

The putative ionophoretic action of phosphatidic acid or arachidonic acid metabolites for Ca2+ has offered an attractive explanation for stimulation-coupled mobilization of cytoplasmic Ca2+. We have examined the effects of Ca2+ ionophore and long-chain unsaturated fatty acids on the translocation of Ca2+ across the liposomal membrane by using Quin II-entrapped liposomes, a sensitive assay system for ionophoresis of Ca2+. A23187 increased Quin II fluorescence intensity corresponding to the translocation of Ca2+ into liposomes. Similar translocation was observed with unsaturated long-chain fatty acids but not with saturated fatty acids. Thus, when phospholipases of cell membrane are activated by certain stimuli, unsaturated long-chain fatty acids are liberated and might mediate the mobilization of cytoplasmic Ca2+.

摘要

磷脂酸或花生四烯酸代谢产物对钙离子的假定离子载体作用，为刺激偶联的细胞质钙离子动员提供了一个有吸引力的解释。我们通过使用包埋有喹啉 II 的脂质体（一种用于钙离子离子载体作用的灵敏检测系统），研究了钙离子载体和长链不饱和脂肪酸对钙离子跨脂质体膜转运的影响。A23187 增加了与钙离子转运到脂质体中相对应的喹啉 II 荧光强度。不饱和长链脂肪酸也观察到了类似的转运，而饱和脂肪酸则没有。因此，当细胞膜的磷脂酶被某些刺激激活时，不饱和长链脂肪酸被释放出来，并可能介导细胞质钙离子的动员。