Ferreira Filipa Santana, Machado Sá da Bandeira Rita Alexandre Dos Santos Soares de Bellegarde, Constantino Cláudia Alexandra Cecílio de Sampaio Ferreira, da Fonseca Ana Maria Teixeira Duarte Cancela, Gomes Joana da Graça Matias, Rodrigues Rúben Miguel Lopes, Atouguia Jorge Luís Marques da Silva, Centeno-Lima Sónia Chavarria Alves Ferreira
Unidade de Clínica Tropical e Centro de Malária e Doenças Tropicais-LA, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Rua da Junqueira 100, 1349-008 Lisboa, Portugal.
J Parasitol Res. 2013;2013:252971. doi: 10.1155/2013/252971. Epub 2013 Sep 8.
Giardia duodenalis is the most prevalent intestinal protozoan infection especially in children. In Portugal scarce data are available relative to this infection in preschoolers. The present study was conducted from April to July 2009 in public preschools in Lisbon enrolling 316 children. Stool examination was performed through microscopy. Molecular analysis was conducted in all positive samples for G. duodenalis in order to determine the assemblage and subassemblage of this parasite. Eight of the preschoolers studied children (2.5%, 8/316) were infected with G. duodenalis. Additionally the brother of one of the infected children was also infected. Genotyping analysis targeting ssu-rRNA and β -giardin loci revealed six infections with assemblage A and 3 with assemblage B. Sub-assemblage determination was possible in four of the samples, with three A2 and one A3. The limited number of cases precluded an association of a determined symptom with an assemblage. The data presented here show the relevance of considering G. duodenalis analysis in children with intestinal complaints even in developed countries.
十二指肠贾第虫是最常见的肠道原生动物感染，尤其是在儿童中。在葡萄牙，关于学龄前儿童这种感染的可用数据很少。本研究于2009年4月至7月在里斯本的公立幼儿园进行，招募了316名儿童。通过显微镜进行粪便检查。对所有十二指肠贾第虫阳性样本进行分子分析，以确定该寄生虫的种群和亚种群。在研究的学龄前儿童中，有8名儿童（2.5%，8/316）感染了十二指肠贾第虫。此外，其中一名感染儿童的兄弟也被感染。针对小亚基核糖体RNA（ssu-rRNA）和β-贾第虫蛋白基因座的基因分型分析显示，6例感染为A种群，3例为B种群。在4个样本中可以确定亚种群，其中3个为A2，1个为A3。病例数量有限，无法确定特定症状与种群之间的关联。此处呈现的数据表明，即使在发达国家，对于有肠道不适的儿童，考虑进行十二指肠贾第虫分析也具有重要意义。
