Stalnikowicz R, Goder K, Karmeli F, Fiocchi C, Rachmilewitz D
Gut. 1985 Jun;26(6):556-61. doi: 10.1136/gut.26.6.556.
The activity of an interferon induced enzyme, (2',5') oligo adenylate synthetase, was determined in peripheral blood mononuclear cells and granulocytes of patients with inflammatory bowel disease and compared with its activity in cells isolated from normal subjects. In spite of the fact that circulating interferon is detected in patients with inflammatory bowel disease, we failed to see any increase in (2',5') oligo adenylate synthetase activity in these patients. The mean +/- SE enzyme activity given in nmol ATP incorporated into (2',5') isoadenylate oligomers by extracts of 10(5) peripheral blood mononuclear cells/21 hours was in normal subjects 1.84 +/- 0.30 (n = 27), in patients with active Crohn's disease 1.38 +/- 0.15 (n = 20) and in patients with active ulcerative colitis 1.14 +/- 0.23 (n = 21). (2',5') oligo adenylate synthetase activity in granulocytes was also similar in normal subjects and in patients with active ulcerative colitis or Crohn's disease. The enzyme activity in patients with active disease was similar both prior to and during steroid therapy. The low (2',5') oligo adenylate synthetase activity in peripheral blood mononuclear cells and granulocytes of patients with active inflammatory bowel disease may reflect decreased cellular response to interferon or a difference in the type of interferon elevated in viral diseases and in inflammatory bowel disease.
在炎症性肠病患者的外周血单核细胞和粒细胞中测定了一种干扰素诱导酶，即（2'，5'）寡腺苷酸合成酶的活性，并将其与从正常受试者分离的细胞中的活性进行了比较。尽管在炎症性肠病患者中检测到循环干扰素，但我们未能在这些患者中观察到（2'，5'）寡腺苷酸合成酶活性有任何增加。10⁵个外周血单核细胞提取物在21小时内掺入（2'，5'）异腺苷酸寡聚物中的ATP的平均±标准误酶活性，正常受试者为1.84±0.30（n = 27），活动性克罗恩病患者为1.38±0.15（n = 20），活动性溃疡性结肠炎患者为1.14±0.23（n = 21）。正常受试者以及活动性溃疡性结肠炎或克罗恩病患者的粒细胞中（2'，5'）寡腺苷酸合成酶活性也相似。活动性疾病患者在类固醇治疗前和治疗期间的酶活性相似。活动性炎症性肠病患者外周血单核细胞和粒细胞中低水平的（2'，5'）寡腺苷酸合成酶活性可能反映了细胞对干扰素的反应降低，或者病毒疾病和炎症性肠病中升高的干扰素类型存在差异。
