Nakasako Masayoshi, Takayama Yuki, Oroguchi Tomotaka, Sekiguchi Yuki, Kobayashi Amane, Shirahama Keiya, Yamamoto Masaki, Hikima Takaaki, Yonekura Koji, Maki-Yonekura Saori, Kohmura Yoshiki, Inubushi Yuichi, Takahashi Yukio, Suzuki Akihiro, Matsunaga Sachihiro, Inui Yayoi, Tono Kensuke, Kameshima Takashi, Joti Yasumasa, Hoshi Takahiko
Department of Physics, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan.
Rev Sci Instrum. 2013 Sep;84(9):093705. doi: 10.1063/1.4822123.
We have developed an experimental apparatus named KOTOBUKI-1 for use in coherent X-ray diffraction imaging experiments of frozen-hydrated non-crystalline particles at cryogenic temperature. For cryogenic specimen stage with small positional fluctuation for a long exposure time of more than several minutes, we here use a cryogenic pot cooled by the evaporation cooling effect for liquid nitrogen. In addition, a loading device is developed to bring specimens stored in liquid nitrogen to the specimen stage in vacuum. The apparatus allows diffraction data collection for frozen-hydrated specimens at 66 K with a positional fluctuation of less than 0.4 μm and provides an experimental environment to easily exchange specimens from liquid nitrogen storage to the specimen stage. The apparatus was developed and utilized in diffraction data collection of non-crystalline particles with dimensions of μm from material and biological sciences, such as metal colloid particles and chloroplast, at BL29XU of SPring-8. Recently, it has been applied for single-shot diffraction data collection of non-crystalline particles with dimensions of sub-μm using X-ray free electron laser at BL3 of SACLA.
我们开发了一种名为KOTOBUKI-1的实验装置，用于在低温下对冷冻水合非晶颗粒进行相干X射线衍射成像实验。对于在几分钟以上的长时间曝光中位置波动较小的低温样品台，我们在此使用通过液氮蒸发冷却效应冷却的低温罐。此外，还开发了一种加载装置，用于将储存在液氮中的样品带到真空中的样品台上。该装置允许在66K下对冷冻水合样品进行衍射数据收集，位置波动小于0.4μm，并提供了一个便于将样品从液氮储存器交换到样品台的实验环境。该装置是在SPring-8的BL29XU上开发并用于收集来自材料和生物科学领域的尺寸为μm的非晶颗粒（如金属胶体颗粒和叶绿体）的衍射数据。最近，它已被应用于在SACLA的BL3上使用X射线自由电子激光对尺寸为亚μm的非晶颗粒进行单次衍射数据收集。
