Lewis R A, Robin J L, Austen K F
Int Arch Allergy Appl Immunol. 1985;77(1-2):121-5. doi: 10.1159/000233765.
Mucosal mast cells constitute the subclass of IgE-FcR-bearing cells for which the murine bone marrow derived mast cell (BMMC) is an in vitro model. BMMC can be induced by an IgE-dependent mechanism to biosynthesize prostaglandin D2, several 5-lipoxygenase products, and an alkyl-ether phospholipid metabolite, as well as to degranulate. By introduction of selective enzyme inhibitors, it was determined that the bioavailability of each mediator class occurred without regulatory effects on the others. Additionally, since BMMC divide in culture, cell responsiveness for secretion of each mediator class was shown to be independent of the state of proliferation by employing three different nontoxic inhibitors of cell division.
黏膜肥大细胞构成了一类带有IgE-FcR的细胞亚群，小鼠骨髓来源的肥大细胞（BMMC）是其体外模型。BMMC可通过IgE依赖机制被诱导生物合成前列腺素D2、几种5-脂氧合酶产物和一种烷基醚磷脂代谢物，以及发生脱颗粒。通过引入选择性酶抑制剂，确定了每类介质的生物利用度的发生对其他介质没有调节作用。此外，由于BMMC在培养中会分裂，通过使用三种不同的无毒细胞分裂抑制剂，表明每类介质分泌的细胞反应性与增殖状态无关。
