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铁基纳米颗粒对假单胞菌 YF1 反硝化的影响。

Impact of iron-based nanoparticles on microbial denitrification by Paracoccus sp. strain YF1.

机构信息

School of Life Science, Fujian Normal University, Fuzhou 350108, China.

出版信息

Aquat Toxicol. 2013 Oct 15;142-143:329-35. doi: 10.1016/j.aquatox.2013.09.005. Epub 2013 Sep 16.

DOI:10.1016/j.aquatox.2013.09.005
PMID:24090609
Abstract

This study investigates the effects of Fe and Fe/Ni nanoparticles on biological denitrification when using Paracoccus sp. strain YF1. Results show that adding Fe and Fe/Ni nanoparticles to the cells decreased their growth and denitrification rate. Compared to that of free cells (control 89.47%), a decrease (64.33%) in the presence of 1000 mg/L Fe/Ni nanoparticles was observed, while a small decline in the denitrification rate (76.36%) was obtained when the concentration of Fe nanoparticles was 1000 mg/L. These were further confirmed by adding Fe(2+), Fe(3+), Fe3O4, Fe(2+)/Ni(2+) and Fe(3+)/Ni(2+) individually to the free cell system. Furthermore, Fe and Fe/Ni nanoparticles influenced the nitrate removal and bacterial growth under different pH and temperature conditions. SEM, XRD and EDS demonstrated that iron oxides formed as a result of nanoparticles corrosion in biological medium. Finally the presence of nanoparticles around some bacteria was observed.

摘要

本研究考察了 Fe 和 Fe/Ni 纳米颗粒对 Paracoccus sp. 菌株 YF1 进行生物反硝化时的影响。结果表明,向细胞中添加 Fe 和 Fe/Ni 纳米颗粒会降低其生长和反硝化速率。与自由细胞(对照 89.47%)相比,当添加 1000mg/L 的 Fe/Ni 纳米颗粒时,观察到反硝化速率下降(64.33%),而当 Fe 纳米颗粒浓度为 1000mg/L 时,反硝化速率略有下降(76.36%)。通过向自由细胞体系中分别添加 Fe(2+)、Fe(3+)、Fe3O4、Fe(2+)/Ni(2+)和 Fe(3+)/Ni(2+)进一步证实了这一点。此外,Fe 和 Fe/Ni 纳米颗粒还影响了不同 pH 值和温度条件下的硝酸盐去除和细菌生长。SEM、XRD 和 EDS 表明,纳米颗粒在生物介质中的腐蚀导致了氧化铁的形成。最后观察到一些细菌周围存在纳米颗粒。

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