Department of Chemistry, Selcuk University, 42075 Konya, Turkey.
Department of Chemistry, Kirklareli University, Kirklareli 39000, Turkey.
Colloids Surf B Biointerfaces. 2014 Jan 1;113:182-9. doi: 10.1016/j.colsurfb.2013.08.019. Epub 2013 Sep 12.
A silica-based β-cyclodextrin was immobilized on magnetic nanoparticles to obtain a macrocyclic compound with magnetic property. Then, the β-cyclodextrin-grafted magnetic nanoparticles were encapsulated with Candida rugosa lipase in sol-gel matrices using alkoxysilane precursors. The catalytic activity of the encapsulated lipases was evaluated with model reactions, i.e., the hydrolysis of p-nitro-phenylpalmitate (p-NPP) and the enantioselective hydrolysis of rasemic Naproxen methyl ester that was studied in an aqueous buffer solution/isooctane reaction system. The results indicate that the cyclodextrin-based, encapsulated lipase particularly exhibited high conversion and enantioselectivity behavior compared to the sol-gel free lipase. It was also observed that excellent enantioselectivity (E=399) was obtained for the encapsulated lipase with magnetic β-cyclodextrin that has an ee value of S-Naproxen acid of about 98%.
一种基于硅的β-环糊精被固定在磁性纳米粒子上，以获得具有磁性的大环化合物。然后，β-环糊精接枝的磁性纳米粒子被用烷氧基硅烷前体包封在溶胶-凝胶基质中的 Candida rugosa 脂肪酶中。用模型反应评估包封脂肪酶的催化活性，即对硝基苯棕榈酸酯（p-NPP）的水解和外消旋 Naproxen 甲酯的对映选择性水解，该反应在水性缓冲溶液/异辛烷反应体系中进行。结果表明，与无溶胶-凝胶的脂肪酶相比，基于环糊精的包封脂肪酶表现出高转化率和对映选择性。还观察到，具有磁性β-环糊精的包封脂肪酶获得了优异的对映选择性（E=399），其 S-Naproxen 酸的ee 值约为 98%。
