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酵母蛋白中磷酸酪氨酸的鉴定以及与质膜相关的一种蛋白酪氨酸激酶的鉴定。

Identification of phosphotyrosine in yeast proteins and of a protein tyrosine kinase associated with the plasma membrane.

作者信息

Castellanos R M, Mazón M J

出版信息

J Biol Chem. 1985 Jul 15;260(14):8240-2.

PMID:2409083
Abstract

[32P]Phosphotyrosine was detected in a hydrolysate of yeast proteins after in vivo labeling with [32P]phosphoric acid. The phosphoamino acid was present in cells exponentially growing on glucose as well as in cells that had reached the stationary phase of growth. Also, a plasma membrane preparation was shown to phosphorylate casein on tyrosine residues.

摘要

在用[32P]磷酸进行体内标记后,在酵母蛋白水解物中检测到了[32P]磷酸酪氨酸。这种磷酸化氨基酸存在于以葡萄糖为碳源指数生长的细胞以及已达到生长稳定期的细胞中。此外,还发现一种质膜制剂能使酪蛋白的酪氨酸残基磷酸化。

相似文献

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Identification of phosphotyrosine in yeast proteins and of a protein tyrosine kinase associated with the plasma membrane.酵母蛋白中磷酸酪氨酸的鉴定以及与质膜相关的一种蛋白酪氨酸激酶的鉴定。
J Biol Chem. 1985 Jul 15;260(14):8240-2.
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Identification of a protein cross-reacting with anti-phosphotyrosine antibodies in yeast insoluble cytoplasmic matrices.在酵母不溶性细胞质基质中鉴定一种与抗磷酸酪氨酸抗体发生交叉反应的蛋白质。
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Protein-tyrosine kinase activity in Saccharomyces cerevisiae.酿酒酵母中的蛋白质酪氨酸激酶活性。
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Identification of GIT1/Cat-1 as a substrate molecule of protein tyrosine phosphatase zeta /beta by the yeast substrate-trapping system.
通过酵母底物捕获系统鉴定GIT1/Cat-1为蛋白酪氨酸磷酸酶ζ/β的底物分子。
Proc Natl Acad Sci U S A. 2001 Jun 5;98(12):6593-8. doi: 10.1073/pnas.041608698. Epub 2001 May 29.
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