Chaby R, Girard R
J Immunol Methods. 1985 Jun 25;80(2):255-65. doi: 10.1016/0022-1759(85)90026-2.
Mouse splenic B cells were found to be rapidly and reproducibly fractionated by adherence at 4 degrees C to plastic dishes pretreated with an alkaline buffer. The cell subsets obtained exhibited marked differences in size, surface immunoglobulins (sIg), and responses to B mitogens. Adherent B lymphocytes, bearing high densities of sIg, responded particularly to LPS whereas non-adherent B lymphocytes, with low densities of sIg, responded especially to dextran sulfate. The correlations between the separated populations and subsets at distinct maturation stages are discussed.
研究发现，通过在4℃下将小鼠脾B细胞贴附于用碱性缓冲液预处理的塑料培养皿上，可快速且可重复地对其进行分离。所获得的细胞亚群在大小、表面免疫球蛋白（sIg）以及对B细胞有丝分裂原的反应方面表现出显著差异。带有高密度sIg的贴壁B淋巴细胞对脂多糖（LPS）反应尤为明显，而带有低密度sIg的非贴壁B淋巴细胞对硫酸葡聚糖反应尤为明显。文中讨论了分离出的群体与不同成熟阶段的亚群之间的相关性。
