Booth R J, Watson J D
J Immunol. 1984 Sep;133(3):1346-9.
Two distinct classes of B cell growth factor (BCGF) have been described. BCGF I induces proliferation of anti-IgM-activated B cells, whereas BCGF II acts on a subpopulation activated by dextran sulfate. We examined the effect on both these B cell subsets of a B cell growth and differentiation factor that copurifies with interleukin 1 (BGDF/IL 1) produced by the WEHI-3 cell line. Proliferation was induced by BGDF/IL 1 in cultures of normal spleen cells depleted of T cells and adherent cells and activated by anti-IgM or dextran sulfate. Little effect was observed in cultures of spleen cells from B cell-deficient CBA/N mice. In cultures of normal B cells activated with anti-IgM and dextran sulfate together, BGDF/IL 1 induced a proliferative response comparable with the sum of the responses to the factor and either stimulus alone. These results suggest that anti-IgM and dextran sulfate activate distinct and independent B cell subpopulations, both of which are responsive to BGDF/IL 1.
已描述了两类不同的B细胞生长因子（BCGF）。BCGF I诱导抗IgM激活的B细胞增殖，而BCGF II作用于由硫酸葡聚糖激活的亚群。我们研究了与WEHI-3细胞系产生的白细胞介素1（BGDF/IL 1）共纯化的一种B细胞生长和分化因子对这两种B细胞亚群的影响。BGDF/IL 1在去除T细胞和贴壁细胞并由抗IgM或硫酸葡聚糖激活的正常脾细胞培养物中诱导增殖。在B细胞缺陷的CBA/N小鼠的脾细胞培养物中观察到的影响很小。在用抗IgM和硫酸葡聚糖共同激活的正常B细胞培养物中，BGDF/IL 1诱导的增殖反应与对该因子和单独任何一种刺激的反应之和相当。这些结果表明，抗IgM和硫酸葡聚糖激活不同且独立的B细胞亚群，这两个亚群均对BGDF/IL 1有反应。
