Veomett G E, Kumor N, Lahm B D
J Interferon Res. 1985 Spring;5(2):327-37. doi: 10.1089/jir.1985.5.327.
The interferon (IFN)-dependent induction of two double-stranded RNA-dependent enzymes was examined in L cells and an L-cell variant (WDIFN) which is highly resistant to the inhibitory effects of IFN on cellular multiplication. IFN, in a concentration-dependent manner, inhibited the multiplication of parental L cells and induced increased levels of the double-stranded RNA-dependent enzymes in parental L cells. Although WDIFN cells were resistant to the antiproliferative effects of IFN, the cells responded to IFN by increasing their levels of the double-stranded RNA-dependent enzymes. However, the level of activity of each enzyme was lower in the WDIFN line than the parental line when both lines were treated with similar concentrations of IFN. The reduced response of the WDIFN line was not the result of the line being a heterogeneous population of cells nor of IFN being more unstable in the presence of WDIFN cells. In addition there was no evidence that WDIFN cells produced a mitogenic factor that could overcome the antiproliferative effects of IFN, nor that sodium butyrate could increase the sensitivity of WDIFN cells to the antiproliferative effects of IFN.
在L细胞和一种对干扰素抑制细胞增殖作用具有高度抗性的L细胞变体（WDIFN）中，研究了干扰素（IFN）依赖性诱导的两种双链RNA依赖性酶。干扰素以浓度依赖的方式抑制亲代L细胞的增殖，并诱导亲代L细胞中双链RNA依赖性酶的水平升高。虽然WDIFN细胞对干扰素的抗增殖作用具有抗性，但这些细胞通过增加双链RNA依赖性酶的水平对干扰素作出反应。然而，当用相似浓度的干扰素处理时，WDIFN系中每种酶的活性水平低于亲代系。WDIFN系反应降低并非该系是细胞异质群体的结果，也不是干扰素在WDIFN细胞存在下更不稳定的结果。此外，没有证据表明WDIFN细胞产生了能克服干扰素抗增殖作用的促有丝分裂因子，也没有证据表明丁酸钠能增加WDIFN细胞对干扰素抗增殖作用的敏感性。
